Methods of removing lipid from a protein and lipid-containing material

Patent metadata

Field	Value
Publication number	US-9560861-B2
Application number	US-49489409-A
Country	US
Kind code	B2
Filing date	Jun 30, 2009
Priority date	Jul 9, 2008
Publication date	Feb 7, 2017
Grant date	Feb 7, 2017

Abstract

Novel methods for producing whey protein concentrates with favorable properties are provided. The methods include using chitosan selective precipitation whey pre-treatment, and microfiltration using polymeric membranes. The products obtained using these methods include WPC80 with low fat content, high clarity, low browning potential during storage, and having low levels of volatiles. Compositions and foaming agents obtained using the methods of the present invention are also provided.

First claim

What is claimed is:

1. A method of removing lipid from a protein and lipid-containing material, the method comprising:
adding sufficient amount of chitosan to the protein and lipid-containing material to form a chitosan-lipid complex in solution, wherein the protein and lipid-containing material is whey;
adjusting the solution to a pH of about 4.0 to 5.0;
mixing the solution for a sufficient time to form a precipitate of the chitosan-lipid complex; and
separating the chitosan-lipid complex from the solution using microfiltration to form a substantially lipid-free protein permeate, wherein the microfiltration is performed with a polymeric microfiltration membrane that has a pore size of from about 0.1 μm to about 1.0 μm, wherein the substantially lipid-free protein permeate comprises β-lactoglobulin.

2. A method of removing lipid from a protein and lipid-containing material, the method comprising:
adding sufficient amount of chitosan to the protein and lipid-containing material to form a chitosan-lipid complex in solution;
adjusting the solution to a pH of about 4.0 to 5.0;
mixing the solution for a sufficient time to form a precipitate of the chitosan-lipid complex; and
separating the chitosan-lipid complex from the solution using microfiltration to form a substantially lipid-free protein permeate, wherein the microfiltration is performed with a polymeric microfiltration membrane having a pore size of from about 0.05 μm to about 5.0 μm.

3. The method of claim 2 , wherein the substantially lipid-free protein permeate is lactose-enriched.

4. The method of claim 2 wherein light absorption of a 5% aqueous solution of the substantially lipid-free whey protein permeate measured at 500 nm is less than 0.06 relative absorbance units.

5. The method of claim 2 wherein the polymeric microfiltration membrane has a pore size of from about 0.1 μm to about 1.0 μm.

6. The method of claim 2 wherein the polymeric microfiltration membrane is a spiral-wound polymeric microfiltration membrane.

7. The method of claim 2 wherein the microfiltration is performed at a temperature of between 5° C. and 55° C.

8. The method of claim 2 wherein the protein and lipid-containing material is whey.

9. The method of claim 8 further comprising clarifying the whey prior to adding chitosan.

10. The method of claim 2 wherein the substantially lipid-free protein permeate comprises β-lactoglobulin.

11. The method of claim 2 , further comprising:
adjusting the substantially lipid-free protein permeate to a pH of from about 6 to about 7 to precipitate unbound chitosan; and
removing the unbound chitosan.

Assignees

• Lucey John A
• Jarto Iswandi
• Smith Karen E
• Damodaran Srinivasan
• Wisconsin Alumni Res Found

Inventors

• Lucey John A
• Jarto Iswandi
• Smith Karen E
• Damodaran Srinivasan

Classifications

• A23L5/273

  using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers · CPC title

• A23J1/205

  from whey, e.g. lactalbumine · CPC title

• A23V2300/40

  Precipitation · CPC title

• A23V2250/54252

  Whey protein · CPC title

• A23C21/00

  Whey; Whey preparations (concentration, evaporation or drying A23C1/00; milk preparations, milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment A23C9/14; preservation of whey or whey preparations A23B11/80) · CPC title