Synbiotic mixture

Patent metadata

Field	Value
Publication number	US-10092606-B2
Application number	US-201514639734-A
Country	US
Kind code	B2
Filing date	Mar 5, 2015
Priority date	Mar 7, 2006
Publication date	Oct 9, 2018
Grant date	Oct 9, 2018

Abstract

This invention relates to a preparation comprising a probiotic bacterial strain and a prebiotic mixture comprising 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of Ga1NAcα1-3Ga1β1-4G1c, Ga1β1-6Ga1Nacα1-3Ga1β1-4G1c and combinations thereof, 20-95 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and 2-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Ga1β1-4G1c and combinations thereof. The invention extends to food products comprising said preparation and to the use of the preparation in the prevention and treatment of infections.

First claim

The invention is claimed as follows:

1. A method for treatment of an immune condition comprising the step of administering to an individual having the immune condition a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of Galactose(“Gal”)-N-acetyl(“NAc”)α1-3Galβ1-4Glucose(“Glc”), Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of Neuraminic acid(“Neu”)Acα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.

2. The method of claim 1 , wherein the oligosaccharide mixture comprises 10-70 wt % of the at least one N-acetylated oligosaccharide, 20-80 wt % of the at least one neutral oligosaccharide and 10-50 wt % of the at least one sialylated oligosaccharide.

3. The method of claim 1 , wherein the prebiotic mixture comprises 15-40 wt % of the at least one N-acetylated oligosaccharide, 40-60 wt % of the at least one neutral oligosaccharide and 15-30 wt % of the at least one sialylated oligosaccharide.

4. The method of claim 1 , wherein the immune condition is selected from the group consisting of food hypersensitivity, eczema, allergic rhinitis, atopic dermatitis, and combinations thereof.

5. A method for treatment of an infection of the upper respiratory tract comprising the step of administering to an individual having the infection of the upper respiratory tract a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of GalNAcα1-3Galβ1-4Glc, Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.

6. The method of claim 5 , wherein the oligosaccharide mixture comprises 10-70 wt % of the at least one N-acetylated oligosaccharide, 20-80 wt % of the at least one neutral oligosaccharide and 10-50 wt % of the at least one sialylated oligosaccharide.

7. The method of claim 5 , wherein the prebiotic mixture comprises 15-40 wt % of the at least one N-acetylated oligosaccharide, 40-60 wt % of the at least one neutral oligosaccharide and 15-30 wt % of the at least one sialylated oligosaccharide.

8. The method of claim 5 , wherein the infection of the upper respiratory tract is otitis media.

9. A method for establishing a bifidogenic intestinal microbiota and/or increasing the metabolic activity of such bifidogenic intestinal microbiota comprising the steps of administering to an individual in need thereof a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of GalNAcα1-3Galβ1-4Glc, Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.

10. The method of claim 9 , wherein the individual is an infant.

11. A method for treatment of a pathogenic infection of the gastro-intestinal tract comprising the step of administering to an individual having the pathogenic infection of the gastro-intestinal tract a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of GalNAcα1-3Galβ1-4Glc, Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.

12. The method of claim 11 , wherein the pathogenic infection is caused by at least one pathogen selected from the group consisting of Staphylococci and Clostridium perfringens.

13. The method of claim 11 , wherein the prebiotic mixture comprises 15-40 wt % of the at least one N-acetylated oligosaccharide, 40-60 wt % of the at least one neutral oligosaccharide and 15-30 wt % of the at least one sialylated oligosaccharide.

14. The method of claim 11 wherein the probiotic bacterial strain is a Lactobacillus strain.

15. The method of claim 14 , wherein the Lactobacillus strain is selected from the group consisting of a Lactobacillus rhamnosus , a Lactobacillus paracasei and a Lactobacillus reuteri.

16. The method of claim 14 , wherein the Lactobacillus strain is Lactobacillus rhamnosus CGMCC 1.3724.

17. The method of claim 14 , wherein the Lactobacillus strain is Lactobacillus paracasei CNCM I-2116.

18. The method of claim 11 , wherein the probiotic bacterial strain is a Bifidobacterium strain.

19. The method of claim 18 , wherein the Bifidobacterium strain is selected from the group consisting of a Bifidobacterium lactis , a Bifidobacterium longum and a Bifidobacterium breve.

20. The method of claim 18 , wherein the Bifidobacterium strain is Bifidobacterium longum ATCC BAA-999.

Assignees

• Nestec Sa

Inventors

• Sprenger Norbert
• Morgan Francois
• Berrocal Rafael
• Braun Marcel
• Cherbut Christine
• Duncan Peter

Classifications

• A61P31/04

  Antibacterial agents · CPC title

• A61K35/745

  Bifidobacteria · CPC title

• A61K47/26

  Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title

• A23C9/1425

  by ultrafiltration, microfiltration or diafiltration of whey, e.g. treatment of the UF permeate · CPC title