Sample analyzing device and sample analyzing method

Patent metadata

Field	Value
Publication number	US-9557326-B2
Application number	US-201113702175-A
Country	US
Kind code	B2
Filing date	Jun 7, 2011
Priority date	Jun 9, 2010
Publication date	Jan 31, 2017
Grant date	Jan 31, 2017

Abstract

This invention provides a sample analyzing device and sample analyzing method designed to suppress nonuniform capture of magnetic particles ( 10 ) and detect a desired substance with higher accuracy. The sample analyzing device includes a flow channel ( 15 ) that conducts thereinto a sample which contains the magnetic particles ( 10 ), and magnetic field generating means ( 12 ) that generates magnetic fields for capturing the magnetic particles ( 10 ) in a magnetic particles capturing region of the flow channel ( 15 ); wherein the flow channel has at least one of structural characteristics that a cross-sectional area of the flow channel, at a downstream end of the magnetic particles capturing region, is larger than a cross-sectional area of the flow channel, at an upstream end of the magnetic particles capturing region, and that the magnetic fields generated by the magnetic field generating means ( 12 ) have a greater magnitude at a downstream side of the magnetic particles capturing region than at an upstream side thereof.

First claim

The invention claimed is:

1. A sample analyzing device comprising:
a flow channel, defined by flow channel walls, that conducts a sample including magnetic particles in a flow direction, at least a portion of the flow channel walls being formed from a transparent material;
a magnet that generates magnetic fields for capturing the magnetic particles in a magnetic particles capturing region located within a transparent flow channel wall section adjacent to the magnet, the magnetic particles capturing region including an upstream side, a downstream side, a length parallel to the flow direction, and a width, perpendicular to the flow direction, that increases linearly from the upstream side to the downstream side, the magnet being disposed in an inclined condition with respect to the flow direction from the upstream side of the magnetic particles capturing region towards the downstream side, and the magnetic fields having a greater magnitude at the downstream side of the magnetic particles capturing region than at the upstream side; and
a detector that detects light transmitted through the magnetic particles capturing region.

2. A sample analyzing device comprising:
a flow channel, defined by flow channel walls, that conducts a sample including magnetic particles in a flow direction, at least a portion of the flow channel walls being formed from a transparent material;
a plurality of magnets that generate magnetic fields for capturing the magnetic particles in a magnetic particles capturing region located within a transparent flow channel wall section adjacent to the magnets, the magnetic particles capturing region including an upstream side, a downstream side, a length parallel to the flow direction, and a width, perpendicular to the flow direction, that increases linearly from the upstream side to the downstream side, the magnets each being disposed to be closer to the magnetic particles capturing region in a direction heading from the upstream side towards the downstream side, and the magnetic fields having a greater magnitude at the downstream side of the magnetic particles capturing region than at the upstream side; and
a detector that detects light transmitted through the magnetic particles capturing region.

3. A sample analyzing device comprising:
a flow channel, defined by flow channel walls, that conducts a sample including magnetic particles in a flow direction, at least a portion of the flow channel walls being formed from a transparent material;
a plurality of electromagnets that generate magnetic fields for capturing the magnetic particles in a magnetic particles capturing region located within a transparent flow channel wall section adjacent to the electromagnets, the magnetic particles capturing region including an upstream side, a downstream side, a length parallel to the flow direction, and a width, perpendicular to the flow direction, that increases linearly from the upstream side to the downstream side, the electromagnets each increasing in a number of windings of a coil in a direction heading from the upstream side of the magnetic particles capturing region towards the downstream side thereof, and the magnetic fields having a greater magnitude at the downstream side of the magnetic particles capturing region than at the upstream side; and
a detector that detects light transmitted through the magnetic particles capturing region.

4. The sample analyzing device according to claim 1 , wherein:
the flow channel is constructed so that the channel cross-sectional area at the magnetic particles capturing region increases monotonously between the upstream side and the downstream side.

5. The sample analyzing device according to claim 1 , wherein:
the magnetic particles capturing region reduces a linear flow velocity of the magnetic particles monotonously between the upstream side and the downstream side.

6. The sample analyzing device according to claim 4 , wherein:
the flow channel is constructed so that a width of the channel, at the magnetic particles capturing region, increases monotonously from the upstream side to the downstream side.

7. The sample analyzing device according to claim 4 , wherein:
the flow channel is constructed so that a height of the channel, at the magnetic particles capturing region, increases monotonously between an upstream side and a downstream side.

8. The sample analyzing device according to claim 1 , wherein: the magnet has a length in the flow direction that is smaller than the length of the magnetic particles capturing region.

9. The sample analyzing device according to claim 8 , wherein: the magnet has an upstream side positioned downstream of the upstream side of the magnetic particles capturing region, and, at the same time, a downstream side positioned upstream of the downstream side of the magnetic particles capturing region.

10. The sample analyzing device according to claim 2 , wherein:
the flow channel is constructed so that the channel cross-sectional area at the magnetic particles capturing region increases monotonously between the upstream side and the downstream side.

11. The sample analyzing device according to claim 2 , wherein:
the magnetic particles capturing region reduces a linear flow velocity of the magnetic particles monotonously between the upstream side and the downstream side.

12. The sample analyzing device according to claim 10 , wherein:
the flow channel is constructed so that a width of the channel, at the magnetic particles capturing region, increases monotonously from the upstream side to the downstream side.

13. The sample analyzing device according to claim 10 , wherein:
the flow channel is constructed so that a height of the channel, at the magnetic particles capturing region, increases monotonously between an upstream side and a downstream side.

14. The sample analyzing device according to claim 2 , wherein: the magnets have a length in the flow direction that is smaller than the length of the magnetic particles capturing region.

15. The sample analyzing device according to claim 14 , wherein: the magnets have an upstream side positioned downstream of the upstream side of the magnetic particles capturing region, and, at the same time, a downstream side positioned upstream of the downstream side of the magnetic particles capturing region.

16. The sample analyzing device according to claim 3 , wherein:
the flow channel is constructed so that the channel cross-sectional area at the magnetic particles capturing region increases monotonously between the upstream side and the downstream side.

17. The sample analyzing device according to claim 3 , wherein:
the magnetic particles capturing region reduces a linear flow velocity of the magnetic particles monotonously between the upstream side and the downstream side.

18. The sample analyzing device according to claim 16 , wherein:
the flow channel is constructed so that a width of the channel, at the magnetic particles capturing region, increases monotonously from the upstream side to the downstream side.

19. The sample analyzing device according to claim 16 , wherein:
the flow channel is constructed so that a height of the channel, at the magnetic particles capturing region, increases monotonously between an upstream side and a downstream side.

20. The sample analyzing device according to claim 3 , wherein: the electromagnets have a length in the flow direction that is smaller than the length of the magnetic particles capturing region, and said electromagnets have an upstrea

Assignees

• Inaba Toru
• Matsuoka Shinya
• Sakazume Taku
• Yamashita Yoshihiro
• Shimada Masafumi
• Kogi Osamu
• Harada Yushi
• Hitachi High Tech Corp

Inventors

• Inaba Toru
• Matsuoka Shinya
• Sakazume Taku
• Yamashita Yoshihiro
• Shimada Masafumi
• Kogi Osamu
• Harada Yushi

Classifications

• G01N33/57525

  of the liver or pancreas · CPC title

• G01N33/5758

  involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites · CPC title

• G01N33/54326

  Magnetic particles · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C1/0332

  using permanent magnets · CPC title