Anti-idiotype antibody against an antibody against the amyloid β peptide

Patent metadata

Field	Value
Publication number	US-9547010-B2
Application number	US-201313960893-A
Country	US
Kind code	B2
Filing date	Aug 7, 2013
Priority date	Dec 22, 2008
Publication date	Jan 17, 2017
Grant date	Jan 17, 2017

Abstract

The present invention is directed to an anti-idiotype antibody binding to the complementary determining region of an antibody against the amyloid β peptide. In one embodiment said antibody binds to the same epitope or an overlapping epitope as the antibody obtainable from the cell line DSM ACC2939. Also reported is an immunoassay for the determination of an antibody against the amyloid β peptide and for determination of an anti-idiotype antibody binding to an antibody against the amyloid β peptide.

First claim

The invention claimed is:

1. A method for the immunological quantification of an anti-amyloid β peptide (anti-Aβ) antibody in a sample, the method comprising the steps of:
providing an immunoassay comprising an antibody obtained from cell line DSM ACC2939, wherein said immunoassay comprises a capture antibody, a tracer antibody and a detection antibody, said tracer antibody is conjugated to a detectable label, wherein at least one of the capture antibody and the tracer antibody is the antibody obtained from cell line DSM ACC2939;
simultaneously or sequentially incubating the capture antibody and the tracer antibody with the sample to form a (capture antibody)-(anti-Aβantibody)-(tracer antibody) complex;
incubating the (capture antibody)-(anti-Aβantibody)-(tracer antibody) complex with the detection antibody, wherein said detection antibody is directed against the detectable label conjugated to the tracer antibody; and
quantifying the amount of said anti-Aβantibody in the sample by determining the presence of the detectable label of the tracer antibody.

2. The method of claim 1 , wherein:
said capture antibody is a biotinylated anti-idiotype antibody against an anti-Aβantibody, is obtained from cell line DSM ACC2939, and is conjugated to a solid phase via streptavidin;
said tracer antibody is an anti-idiotype antibody against an anti-Aβantibody, is obtained from cell line DSM AC2939, and is conjugated to digoxygenin as the detectable label; and
said detection antibody is directed against digoxygenin and is conjugated to a peroxidase.

3. The method of claim 1 , wherein said capture antibody is a mixture comprising at least two of said capture antibodies that differ in the antibody site at which they are conjugated to the solid phase, and the tracer antibody is a mixture comprising at least two of said tracer antibodies that differ in the antibody site at which they are conjugated to the detectable label.

4. A method for the immunological quantification of an anti-amyloid β peptide (anti-Aβ) antibody in a sample, the method comprising the steps of:
providing an immunoassay comprising an anti-idiotype antibody against an anti-Aβ antibody, wherein the anti-idiotype antibody is obtained from cell line DSM ACC2939, and wherein said immunoassay comprises amyloid-βpeptide conjugated to a solid phase, the anti-idiotype antibody obtained from cell line DSM ACC2939 conjugated to digoxygenin as a detectable label, and a peroxidase-conjugated detection antibody directed against digoxygenin;
simultaneously or sequentially incubating the amyloid-βpeptide, the sample and the anti-idiotype antibody together to form a (Aβpeptide)-(anti-Aβantibody)-(anti-idiotype antibody) complex;
incubating the (Aβpeptide)-(anti-Aβantibody)-(anti-idiotype antibody) complex with the detection antibody; and
quantifying the amount of said anti-Aβantibody in the sample by determining the presence of the detectable label of the detection antibody.

Assignees

• Hoffmann La Roche

Inventors

• Essig Ulrich
• Hoesel Wolfgang
• Stubenrauch Kay-Gunnar
• Vogel Rudolf

Classifications

• C07K16/4241 Primary

  against anti-human or anti-animal Ig · CPC title

• C07K2317/56

  variable (Fv) region, i.e. VH and/or VL · CPC title

• G01N2469/20

  Detection of antibodies in sample from host which are directed against antigens from microorganisms · CPC title

• G01N2333/4709

  Amyloid plaque core protein · CPC title

• C07K16/42 Primary

  against immunoglobulins · CPC title