Compositions, kits, and methods for isolating vesicles

Patent metadata

Field	Value
Publication number	US-9546938-B2
Application number	US-201313857087-A
Country	US
Kind code	B2
Filing date	Apr 4, 2013
Priority date	Sep 28, 2012
Publication date	Jan 17, 2017
Grant date	Jan 17, 2017

Abstract

A composition, a kit, and a method of isolating a vesicle from a sample using a compound comprising zwitterion moieties, which may be used to analyze vesicles, and proteins, glycoprotein, lipids, or nucleic acids thereof.

First claim

What is claimed is:

1. A method of isolating a vesicle from a sample, the method comprising:
incubating a compound comprising zwitterionic moieties with a sample comprising a vesicle to form a reaction mixture wherein the compound binds to the vesicle; and
isolating the vesicle from the reaction mixture,
wherein the sample is a body fluid sample or cell culture sample from which a cell or a cell residue has been removed.

2. The method of claim 1 , wherein the sample is urine, mucus, saliva, tears, blood plasma, serum, sputum, spinal fluid, hydrothorax, nipple aspirate, lymph fluid, airway fluid, intestinal fluid, genitourinary fluid, breast milk, fluid in lymphatic system, semen, cerebrospinal fluid, fluid in organ system, ascites, cystic neoplasm fluid, amniotic fluid, or a combination thereof.

3. The method of claim 1 , wherein the compound comprising zwitterionic moieties is fixed on a solid support.

4. The method of claim 1 , further comprising detecting the isolated vesicle.

5. The method of claim 1 , wherein the zwitterionic moieties have the following formula:

wherein A 1 and A 2 are functional groups having electrical charges that are opposite to each other;
R 1 is an aliphatic hydrocarbon or an aromatic hydrocarbon;
R 2 is an amine, a carboxyl group, or a hydroxyl group;
n is from 1 to 100; and
m is from 1 to 100.

6. The method of claim 1 , wherein the zwitterionic moieties are sulfobetaine (SB), carboxybetaine, phosphorylcholine, or a combination thereof.

7. The method of claim 1 , wherein the compound comprising zwitterionic moieties is a polymer comprising zwitterionic moieties.

8. The method of claim 3 , wherein the solid support is a magnetic bead, a silica bead, a polystyrene plate, a polystyrene bead, a glass bead, a cellulose bead, or a combination thereof.

9. The method of claim 1 , wherein the vesicle is a liposome or a microvesicle.

10. The method of claim 5 , wherein A 1 is ammonium, sulfonium, or phosphonium, and A 2 is carboxylic acid, phosphoric acid, or phosphonic acid; or A 1 is carboxylic acid, phosphoric acid, or phosphonic acid, and A 2 is ammonium, sulfonium, or phosphonium.

11. The method of claim 5 , wherein R 1 is a C 1 -C 8 aliphatic hydrocarbon; R 2 is a carboxyl group; n is from about 1 to about 10; and m is from about 1 to about 10.

12. The method of claim 10 , wherein R 1 is a C 1 -C 8 aliphatic hydrocarbon; R 2 is a carboxyl group; n is from about 1 to about 10; and m is from about 1 to about 10.

13. The method of claim 11 , wherein R 1 is a C 1-8 alkane.

14. The method of claim 12 , wherein R 1 is a C 1-8 alkane, a C 1-8 alkene, or a C 1-8 alkyne.

15. The method of claim 1 , wherein the vesicle comprises a nucleic acid.

16. The method of claim 1 , wherein the vesicle comprises miRNA.

17. The method of claim 15 , wherein the method further comprises analyzing the nucleic acid.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Kim Ga-Hee
• Yoo Chang-Eun
• Yong Ye-Ryoung
• Lee Myo-Yong

Classifications

• G01N33/5759

  involving compounds localised on the membrane of tumour or cancer cells · CPC title

• G01N1/405 Primary

  by adsorption or absorption · CPC title

• C12Q1/6886

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• G01N33/5076 Primary

  involving cell organelles, e.g. Golgi complex, endoplasmic reticulum · CPC title

• G01N33/57492

  Physics · mapped topic