Variant LovD polypeptides and their uses
US-9181570-B2 · Nov 10, 2015 · US
Variant LovD polypeptides and their uses
US9546388B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9546388-B2 |
| Application number | US-201514710893-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 13, 2015 |
| Priority date | Sep 30, 2009 |
| Publication date | Jan 17, 2017 |
| Grant date | Jan 17, 2017 |
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- Title
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Abstract
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The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of making simvastatin comprising contacting monacolin J or lovastatin substrate with a variant LovD polypeptide having at least 90% sequence identity to the wild-type Aspergillus terreus acyltransferase of SEQ ID NO:2, wherein said variant comprises the substitution L361M and from 1 to 30 additional substitutions, and wherein said contacting is conducted in the presence of an α-dimethylbutyryl thioester co-substrate and under conditions in which simvastatin is produced. 2. The method of claim 1 , wherein the monacolin J is a sodium salt. 3. The method of claim 2 , wherein the monacolin J is a sodium salt and activated charcoal is added. 4. The method of claim 1 , wherein the monacolin J is an ammonium salt. 5. The method of claim 4 , wherein an agent for precipitating simvastatin is added. 6. The method of claim 5 , wherein said agent is ammonium hydroxide. 7. The method of claim 1 , wherein said lovastatin substrate, said variant LovD polypeptide and said thioester are added at substantially the same time into a vessel. 8. The method of claim 7 , wherein said lovastatin substrate and said variant LovD polypeptide are first added into a vessel and then said thioester is added into said vessel. 9. The method of claim 1 , wherein an agent for precipitating simvastatin is added. 10. The method of claim 9 , wherein said agent is ammonium hydroxide.
Assignees
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Classifications
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title
- C12P17/06Primary
containing a six-membered hetero ring, e.g. fluorescein · CPC title
Carboxylic acid esters · CPC title
Acyltransferases (2.3) · CPC title
transferring groups other than amino-acyl groups (2.3.1) · CPC title
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Frequently asked questions
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- What does patent US9546388B2 cover?
- The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P17/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 17 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).