Aminoacyl tRNA synthetases for modulating inflammation

Patent metadata

Field	Value
Publication number	US-9540628-B2
Application number	US-201414541792-A
Country	US
Kind code	B2
Filing date	Nov 14, 2014
Priority date	Dec 11, 2009
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

Inflammatory and other cellular response-modulating compositions are provided comprising aminoacyl-tRNA synthetase polypeptides, including active fragments and/or variants thereof. Also provided are methods of using such compositions in the treatment of conditions that benefit from the modulation of inflammation, such as inflammatory diseases or conditions.

First claim

The invention claimed is:

1. A composition comprising: a modified mRNA polynucleotide encoding a histidyl tRNA synthetase (HRS) polypeptide and a physiologically-acceptable carrier, where the HRS polypeptide is selected from (a) SEQ ID NO:28, (b) a fragment of SEQ ID NO:28 comprising at least 400 contiguous amino acids of SEQ ID NO:28, and (c) a variant which differs from SEQ ID NO: 28 by less than 10% of the residues of SEQ ID NO: 28, where the modified mRNA polynucleotide is operably linked to a heterologous translational regulatory element.

2. The composition of claim 1 , which is an aqueous solution suitable for intravenous administration to a mammalian subject in need thereof.

3. The composition of claim 1 , comprising liposomes, nanocapsules, microparticles, microspheres, lipid particles, or vesicles.

4. The composition of claim 1 , which is formulated for delivery encapsulated in a lipid particle, a liposome, a vesicle, nanosphere, or a nanoparticle.

5. The composition of claim 1 , where the heterologous translational regulatory element comprises an initiation signal.

6. The composition of claim 1 , where (b) is a fragment of SEQ ID NO:28 comprising at least 500 contiguous amino acids of SEQ ID NO:28.

7. The composition of claim 1 , where (c) is a variant which differs from SEQ ID NO:28 by less than 5% of the residues of SEQ ID NO:28.

Assignees

• Atyr Pharma Inc

Inventors

• Watkins Jeffry Dean
• Vasserot Alain Philippe
• Greene Leslie Ann
• Adams Ryan Andrew
• Chiang Kyle P
• Zhang Wei
• Piehl Kristi Helen
• Hong Fei
• He Alina

Classifications

• A61P37/00

  Drugs for immunological or allergic disorders · CPC title

• A61P37/04

  Immunostimulants · CPC title

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61P39/06

  Free radical scavengers or antioxidants · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title