Spontaneously formed terminal supraparticles having nanoparticles for protein stabilization

Patent metadata

Field	Value
Publication number	US-9534213-B2
Application number	US-201514635347-A
Country	US
Kind code	B2
Filing date	Mar 2, 2015
Priority date	Mar 4, 2014
Publication date	Jan 3, 2017
Grant date	Jan 3, 2017

Abstract

Supraparticle nanoassemblies are provided that comprise nanoparticle species and protein species, as well as methods for making such assemblies. A supraparticle may comprise a nanoparticle species with a first charge and an average particle size diameter of ≧about 1 nm to ≦about 100 nm. The supraparticle also comprises a protein species. The nanoparticle species and the protein species have the same charge and are assembled together without any intramolecular chemical bonding to form the supraparticle. The supraparticle may be a substantially round particle. In certain other aspects, a photoreactive supraparticle is provided, where the nanoparticle is reactive to energy or electromagnetic radiation, which in the presence of such energy or radiation enhances reactivity of the protein species in the supraparticle.

First claim

What is claimed is:

1. A supraparticle comprising:
a nanoparticle species having a surface with a first charge and an average particle size diameter of greater than or equal to about 1 nm to less than or equal to about 100 nm; and
a protein species having a second charge that is the same as the first charge, wherein the nanoparticle species and the protein species are self-assembled together via intermolecular forces without any intramolecular chemical bonding to form the supraparticle having a ζ-potential of greater than or equal to about +30 mV.

2. The supraparticle of claim 1 , wherein the nanoparticle species is selected from the group consisting of: gold (Au), silver (Ag), copper (Cu), nickel (Ni), iron (Fe), carbon (C), platinum (Pt), silicon (Si), cadmium (Cd), cadmium telluride (CdTe), cadmium selenide (CdSe), cadmium sulfide (CdS), mercury tellurium (HgTe), mercury selenide (HgSe), mercury sulfide (HgS), lead telluride (PbTe), lead selenide (PbSe), lead sulfide (PbS), molybdenum sulfide (MoS 2 ), iron (II) sulfide (FeS 2 ), iron sulfide (FeS), iron selenide (FeSe), zinc oxide (ZnO), and combinations thereof.

3. The supraparticle of claim 1 , wherein the nanoparticle species comprises a semiconductive material selected from the group consisting of: Cd, CdS, CdSe, CdTe, Si, and combinations thereof.

4. The supraparticle of claim 1 , wherein the nanoparticle species comprises CdTe or FeS 2 .

5. The supraparticle of claim 1 , wherein the surface of the nanoparticle species comprises at least one stabilizer having the first charge.

6. The supraparticle of claim 5 , wherein the at least one stabilizer comprises 2-(dimethylamino)ethanethiol (DMAET).

7. The supraparticle of claim 6 , wherein the nanoparticle species comprises CdTe, forming a DMAET-stabilized CdTe.

8. The supraparticle of claim 1 , wherein the protein species comprises an enzyme.

9. The supraparticle of claim 8 , wherein the enzyme retains at least 90% of catalytic capability when incorporated into the supraparticle as compared to catalytic capability of the enzyme in a free state.

10. The supraparticle of claim 1 , wherein the supraparticle further comprises a biomolecule.

11. The supraparticle of claim 1 , wherein the first charge and the second charge are positive.

12. The supraparticle of claim 1 , wherein a ratio of the nanoparticle species to the protein species in the supraparticle is about 10:1 to about 1:10.

13. The supraparticle of claim 12 , wherein the ratio of the nanoparticle species to the protein species in the supraparticle is about 1.5:1 to about 1:1.5.

14. The supraparticle of claim 1 , having an average particle size diameter of greater than or equal to about 50 nm to less than or equal to about 200 nm.

15. A supraparticle comprising:
a nanoparticle species having a surface with a first charge and an average particle size diameter of greater than or equal to about 1 nm to less than or equal to about 100 nm; and
a protein species having a second charge that is the same as the first charge, the protein species comprising an enzyme, wherein the nanoparticle species and the protein species are self-assembled together via intermolecular forces without any intramolecular chemical bonding to form the supraparticle having a substantially round shape and a ζ-potential of greater than or equal to about 30 mV, wherein the enzyme retains at least 90% of catalytic capability when incorporated into the supraparticle as compared to catalytic capability of the enzyme in a free state.

16. A photoreactive supraparticle comprising:
a nanoparticle species having a surface with a first charge and an average particle size diameter of greater than or equal to about 1 nm to less than or equal to about 100 nm, wherein the nanoparticle species is reactive to electromagnetic radiation and comprises a material selected from the group consisting of: Cd, CdS, CdSe, CdTe, Si, FeS 2 , and combinations thereof; and
a protein species having a second charge that is the same as the first charge, wherein the nanoparticle species and the protein species are assembled together without any intramolecular chemical bonding to form the supraparticle, wherein in the presence of the electromagnetic radiation, the protein species in the supraparticle has a reactivity that is at least 100% greater than a comparative reactivity of the protein species in the absence of the electromagnetic radiation.

17. The supraparticle of claim 16 , wherein the nanoparticle species comprises CdTe or FeS 2 .

Assignees

• Univ Michigan Regents

Inventors

• Kotov Nicholas A

Classifications

• C12N9/96 Primary

  Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title