Aligned nanofibers and related methods of use

Patent metadata

Field	Value
Publication number	US-9512404-B2
Application number	US-201414325495-A
Country	US
Kind code	B2
Filing date	Jul 8, 2014
Priority date	Feb 14, 2007
Publication date	Dec 6, 2016
Grant date	Dec 6, 2016

Abstract

The present invention relates to nanofibers. In particular, the present invention provides aligned nanofiber bundle assemblies. In some embodiments, the aligned nanofiber bundle assemblies are used for tissue regeneration, controlled growth of cells, and related methods (e.g., diagnostic methods, research methods, drug screening).

First claim

We claim:

1. A method of aligning nanofibers of self-assembling peptide amphiphile molecules; wherein said peptide amphiphile molecules comprise a hydrophobic tail, a peptide segment configured for β-sheet formation, and a charged segment; comprising:
increasing the temperature of said nanofibers in solution from room temperature to 50° C.;
maintaining the temperature of said nanofibers for 20 minutes; and
reducing the temperature of said nanofibers in solution from 50° C. to room temperature.

2. A method of claim 1 , wherein the aligned nanofibers comprise longitudinally-aligned bundles of 15-35 nanofibers.

3. The method of claim 1 , wherein said hydrophobic tail comprises an alkyl tail.

4. The method of claim 3 , wherein said alkyl tail comprises at least 16 carbon atoms.

5. The method of claim 1 , wherein said peptide segment configured for β-sheet formation comprises uncharged amino acid residues.

6. The method of claim 1 , wherein the temperature of said nanofibers is increased to 80° C.

7. The method of claim 1 , wherein the temperature of said nanofibers is maintained for 30 minutes.

8. The method of claim 1 , wherein said peptide segment configured for β-sheet formation comprises three alanine and three valine amino acid residues.

9. The method of claim 1 , wherein said charged segment comprises a charged peptide segment comprising charged amino acid residues.

10. The method of claim 9 , wherein said charged amino acid residues comprises three glutamic acid residues.

11. The method of claim 1 , wherein said aligned nanofibers have a noodle-like formation.

Assignees

• Univ Northwestern

Inventors

• Stupp Samuel I
• Zhang Shuming
• Mata Alvaro
• Greenfield Megan A

Classifications

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12N2533/50

  Proteins · CPC title

• C12N5/0662 Primary

  Stem cells · CPC title