Method for producing glucaric acid

Patent metadata

Field	Value
Publication number	US-9506091-B2
Application number	US-201314377559-A
Country	US
Kind code	B2
Filing date	Feb 19, 2013
Priority date	Feb 24, 2012
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

The disclosure provides a method for creating a transformant having significantly improved glucaric acid-producing capability and a method for efficiently producing glucaric acid using the transformant.

First claim

The invention claimed is:

1. A method of producing glucaric acid comprising:
(1) providing a Escherichia coli strain transformed with an overexpression vector comprising a gene encoding inositol monophosphatase, and having genes encoding inositol-1-phospahte synthase, myo-inositol oxygenase, and uronate dehydrogenase;
(2) growing and/or maintaining the transformed Escherichia coli strain with a carbon source under conditions suitable to convert the carbon source to glucaric acid and/or glucarate; and
(3) separating the glucaric acid or glucarate from the transformed Escherichia coli strain.

2. The method according to claim 1 , wherein the carbon source contains a compound that can be converted into glucose-6-phosphate within the transformed Escherichia coli strain.

3. The method according to claim 2 , wherein the carbon source is one or more selected from the group consisting of D-glucose, sucrose, oligosaccharide, polysaccharide, starch, cellulose, rice bran, molasses, and biomass containing D-glucose.

Assignees

• Asahi Kasei Chemicals Corp

Inventors

• Konishi Kazunobu
• Imazu Shinichi

Classifications

• C12N9/16

  acting on ester bonds (3.1) · CPC title

• C12Y301/03025

  Inositol-phosphate phosphatase (3.1.3.25) · CPC title

• C12P7/58 Primary

  Aldonic, ketoaldonic or saccharic acids (uronic acids C12P19/00) · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title