Endotoxin adsorbent

Patent metadata

Field	Value
Publication number	US-9505850-B2
Application number	US-201314404479-A
Country	US
Kind code	B2
Filing date	May 29, 2013
Priority date	May 30, 2012
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

A means for selectively removing ET under coexistence of a substance showing a negative charge, such as nucleic acid is described. Endotoxin is selectively removed by bringing a polymer obtained by crosslinking cyclodextrin with an isocyanate-based crosslinking agent in contact with a solution containing endotoxin and the substance showing the negative charge such as nucleic acid.

First claim

What is claimed is:

1. A method for removing endotoxin, comprising bringing a solution containing an objective material showing a negative charge and endotoxin into contact with a polymer of cyclodextrin or an adsorbent containing a base material and the polymer as immobilized to the base material,
wherein the polymer has features (1) to (4) described below:
(1) N/C shown according the following formula is 6 to 15;
N/C(mol %)=nitrogen content(mol)/carbon content(mol)×100  (formula 1)

(2) part of hydroxy group of cyclodextrin forms a urethane bond;
(3) insoluble in water; and
(4) anion exchange capacity is less than 0.1 meq/g.

2. A method for producing a solution containing an objective material showing a negative charge from which endotoxin is removed, comprising bringing a solution containing an objective material showing a negative charge and endotoxin into contact with a polymer of cyclodextrin or an adsorbent containing a base material and the polymer as immobilized to the base material,
wherein the polymer has features (1) to (4) described below:
(1) N/C shown according the following formula is 6 to 15;
N/C(mol %)=nitrogen content(mol)/carbon content(mol)×100  (formula 1)

(2) part of hydroxy group of cyclodextrin forms a urethane bond;
(3) insoluble in water; and
(4) anion exchange capacity is less than 0.1 meq/g.

3. The method according to claim 1 , wherein the objective material showing the negative charge is nucleic acid.

4. The method according to claim 3 , wherein the nucleic acid is DNA.

5. The method according to claim 3 , wherein the nucleic acid is RNA.

6. The method according to claim 2 , wherein the objective material showing the negative charge is nucleic acid.

7. The method according to claim 6 , wherein the nucleic acid is DNA.

8. The method according to claim 6 , wherein the nucleic acid is RNA.

9. The method according to claim 1 , wherein the solution is brought into contact with the polymer or the absorbent at pH 4 to 6.

10. The method according to claim 2 , wherein the solution is brought into contact with the polymer or the absorbent at pH 4 to 6.

Assignees

• Nat Univ Corp Kumamoto Univ
• Jnc Corp

Inventors

• Sakata Masayo
• Todokoro Masami

Classifications

• C08B37/0012 Primary

  Cyclodextrin [CD], e.g. cycle with 6 units (alpha), with 7 units (beta) and with 8 units (gamma), large-ring cyclodextrin or cycloamylose with 9 units or more; Derivatives thereof · CPC title

• B01J2220/52

  Sorbents specially adapted for preparative chromatography · CPC title

• B01J20/267 Primary

  Cross-linked polymers · CPC title

• B01J20/3085

  Chemical treatments not covered by groups B01J20/3007 - B01J20/3078 · CPC title

• C08L5/16

  Cyclodextrin; Derivatives thereof · CPC title