Analytical tool and method for determining a condition of an oral cavity

Patent metadata

Field	Value
Publication number	US-9500649-B2
Application number	US-201113976643-A
Country	US
Kind code	B2
Filing date	Dec 27, 2011
Priority date	Dec 28, 2010
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

A method of determining the condition of the oral cavity in a subject is provided by using an analytical tool comprising the following (A), (B), and (C):
(A) a reagent for measuring one or more parameters that reflect the dental caries risk for a test sample obtained from the oral cavity, (B) a reagent for measuring one or more parameters that reflect the periodontal disease risk for the test sample obtained from the oral cavity, and (C) a reagent for measuring one or more parameters that reflect the degree of oral cleanliness for the test sample obtained from the oral cavity.

First claim

The invention claimed is:

1. A method of determining a condition of an oral cavity in a subject, the method comprising the following (A), (B) and (C):
(A) determining a risk level of dental carries by measuring parameters comprising mutans bacteria count, pH and acid buffering ability from a test sample obtained from the oral cavity;
(B) determining a risk level of periodontal disease by measuring parameters comprising total protein concentration, occult blood amount and leukocyte count from the test sample;
(C) determining a degree of oral cleanliness by measuring parameters comprising ammonia concentration and total protein concentration from the test sample,
wherein (A), (B), and (C) are performed using a test piece as an analytical tool, the test piece comprising the following (a), (b), (c), (d), (e), (f) and (g):
(a) an absorptive carrier that contains a reagent for measuring mutans bacteria count;
(b) an absorptive carrier that contains a reagent for measuring pH;
(c) an absorptive carrier that contains a reagent for measuring acid buffering ability;
(d) an absorptive carrier that contains a reagent for measuring total protein concentration;
(e) an absorptive carrier that contains a reagent for measuring occult blood amount;
(f) an absorptive carrier that contains a reagent for measuring leukocyte count; and
(g) an absorptive carrier that contains a reagent for measuring ammonia concentration,
wherein each of (a), (b), (c), (d), (e), (f) and (g) is supported by a single support carrier, and wherein the plural determinations (A), (B) and (C) result in a comprehensive diagnosis of the condition of the oral cavity during a single examination of the subject.

2. The method according to claim 1 further comprising displaying one or more of the determined levels.

3. The method according to claim 1 further comprising displaying a comment based on one or more of the determined levels.

4. The method according to claim 1 ,
wherein each of the parameters is measured by a color reaction,
wherein the reagent for measuring mutans bacteria count comprises resazurin, where reaction time for the mutans bacteria count measurement is 1 to 10 minutes,
wherein the reagent for measuring pH comprises a pH indicator, where reaction time for the pH measurement is 30 seconds to 5 minutes,
wherein the reagent for measuring acid buffering ability comprises an acid buffer and a pH indicator, where reaction time for the acid buffering ability measurement is 30 seconds to 5 minutes,
wherein the reagent for measuring total protein concentration comprises a pH indicator, where reaction time for the total protein concentration measurement is 30 seconds to 5 minutes,
wherein the reagent for measuring occult blood amount comprises a peroxide and an indicator that changes color upon oxidation, where reaction time for the occult blood amount measurement is 30 seconds to 5 minutes,
wherein the reagent for measuring leukocyte count comprises a diazonium salt, where reaction time for the leukocyte count measurement is 30 seconds to 5 minutes, and
wherein the reagent for measuring ammonia concentration comprises boric acid buffering agent and a pH indicator, where reaction time for the ammonia concentration measurement is 10 seconds to 5 minutes.

5. The method according to claim 1 , wherein the reagent for measuring mutans bacteria count comprises resazurin.

6. The method according to claim 5 , wherein the reagent for measuring mutans bacteria count further comprises 1-methoxy-5-methylphenazinium methosulfate.

7. The method according to claim 1 , wherein the reagent for measuring pH comprises a pH indicator.

8. The method according to claim 7 , wherein the pH indicator comprises bromocresol green and bromoxylenol blue.

9. The method according to claim 1 , wherein the reagent for measuring occult blood comprises a peroxide and an indicator that chances color upon oxidation.

10. The method according to claim 1 , wherein the reagent for measuring leukocyte count comprises a diazonium salt.

11. The method according to claim 1 , wherein the reagent for measuring total protein concentration comprises a pH indicator.

12. The method according to claim 11 , wherein the pH indicator comprises tetrabromophenol blue (TBPB), tetrabromophenolphthalein, or 5′,5″-dinitro-3′,3″-diiodo-3,4,5,6-tetrabromophenol sulfophthalein (DIDNTB).

13. The method according to claim 1 , wherein the reagent for measuring ammonia concentration comprises a boric acid buffering agent and a pH indicator.

14. The method according to claim 13 , wherein the pH indicator comprises bromcresol green, bromcresol purple or chlorophenol red.

Assignees

• Nishinaga Eiji
• Uchiyama Akira
• Suzuki Naho
• Fukasawa Tetsu
• Maki Riichi
• Okubo Akio
• Fukuta Isao
• Arkray Inc

Inventors

• Nishinaga Eiji
• Uchiyama Akira
• Suzuki Naho
• Fukasawa Tetsu
• Maki Riichi
• Okubo Akio
• Fukuta Isao

Classifications

• G01N2800/18

  Dental and oral disorders · CPC title

• G01N33/54386

  Analytical elements · CPC title

• C12Q1/689

  for bacteria · CPC title

• C12Q2600/118

  Prognosis of disease development · CPC title

• G01N33/5302

  Apparatus specially adapted for immunological test procedures · CPC title