Compositions and methods for characterizing and diagnosing periodontal disease

Patent metadata

Field	Value
Publication number	US-11713478-B2
Application number	US-202017008282-A
Country	US
Kind code	B2
Filing date	Aug 31, 2020
Priority date	Jun 6, 2014
Publication date	Aug 1, 2023
Grant date	Aug 1, 2023

Abstract

The present disclosure relates to methods for characterizing and diagnosing dental diseases. In particular, the present disclosure relates to methods for characterizing and diagnosing dental disease (e.g., gingivitis and periodontal disease) based on levels of AI-2 in oral fluids and plaque.

First claim

The invention claimed is:

1. A method of treating periodontal disease, comprising;
a) detecting: i) an altered level of Autoinducer-2 (AI-2) in an oral sample; and
ii) the presence of one or more bacteria in said oral sample,

wherein said bacteria are one or more species selected from the group consisting of Akkermansia muciniphila, Coprobacter fastidiosus, Ruminococcus gnavus, Blautia schinkii, Abiotrophia defective, Flavobacteriaceae, Oribacterium sinus , and Bergeyella sp. AF14,
b) diagnosing the presence of periodontal disease when said level of AI-2, and said bacteria are altered relative to the level in subjects not diagnosed with periodontal disease; and
c) administering a treatment for periodontal disease when said altered level of AI-2 are detected.

2. The method of claim 1 , wherein said treatment is selected from an antibiotic, a dental cleaning, and a laser treatment.

3. The method of claim 1 , wherein said treatment is in a mouthwash or toothpaste.

4. The method of claim 1 , further comprising the step of repeating said detecting step one or more times.

5. The method of claim 1 , wherein said oral sample is plaque and said bacteria are one or more species selected from the group consisting of Akkermansia muciniphila, Coprobacter fastidiosus, Ruminococcus gnavus, Blautia schinkii, Abiotrophia defective , and Flavobacteriaceae.

6. The method of claim 1 , wherein said oral sample is saliva and said bacteria are one or more species selected from the group consisting of Akkermansia muciniphila, Bergeyella sp. AF14, and Ruminococcus gnavus.

7. The method of claim 1 , wherein said one or more species are 5 or more species.

8. The method of claim 1 , wherein said one or more species are all of said species.

Assignees

• Univ Michigan Regents
• Colgate Palmolive Co

Inventors

• Rickard Alexander
• Giannobile William
• Swetky Michelle
• Baxter Nielson T
• Miller Steven E
• Du-Thumm Laurence
• Stettler Hans

Classifications

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12Q1/18 Primary

  Testing for antimicrobial activity of a material · CPC title

• C12Q1/66

  involving luciferase · CPC title

• G01N33/56955

  involved in periodontal diseases · CPC title

• G16H50/30

  for calculating health indices; for individual health risk assessment · CPC title