Glucose dehydrogenase

Patent metadata

Field	Value
Publication number	US-9487758-B2
Application number	US-201414176701-A
Country	US
Kind code	B2
Filing date	Feb 10, 2014
Priority date	Aug 11, 2011
Publication date	Nov 8, 2016
Grant date	Nov 8, 2016

Abstract

An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.

First claim

The invention claimed is:

1. An electrode comprising a flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 90% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity, wherein the flavin-binding glucose dehydrogenase is immobilized onto the electrode.

2. The electrode of claim 1 , wherein the polypeptide has an amino acid sequence with 95% or more identity to the amino acid sequence of SEQ ID NO: 1.

3. The electrode of claim 1 , wherein the polypeptide has an amino acid sequence with 98% or more identity to the amino acid sequence of SEQ ID NO: 1.

4. The electrode of claim 1 , wherein the flavin-binding glucose dehydrogenase further has at least one of the following characteristics (1) to (8):
(1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 70 kDa as measured by SDS-polyacrylamide electrophoresis;
(2) Km value: the Km value for D-glucose is about 20 mM or less;
(3) Substrate specificity: the reactivity to D-xylose is 1.7% or less, based on the reactivity to D-glucose taken as 100%;
(4) Optimal activity temperature: 37 to 45° C.;
(5) Optimal activity pH: 6.5;
(6) pH stability: stable at a pH range of 5 to 7;
(7) Temperature stability: stable at a temperature of 40° C. or less; and
(8) Origin: the enzyme is derived from microorganisms of the genus Circinella.

5. A sensor comprising the electrode of claim 1 .

Assignees

• Toyo Boseki

Inventors

• Sumida Yosuke
• Aiba Hiroshi
• Kawaminami Hiroshi
• Hirao Rie
• Utashima Yuu
• Kishimoto Takahide
• Yanagidani Shusaku

Classifications

• C12Y101/05

  with a quinone or similar compound as acceptor (1.1.5) · CPC title

• C12Q1/54

  involving glucose or galactose · CPC title

• C12N9/0006 Primary

  acting on CH-OH groups as donors (1.1) · CPC title