Process for obtaining immunoglobulins from colostral milk

Patent metadata

Field	Value
Publication number	US-9475860-B2
Application number	US-201414168586-A
Country	US
Kind code	B2
Filing date	Jan 30, 2014
Priority date	Jan 31, 2013
Publication date	Oct 25, 2016
Grant date	Oct 25, 2016

Abstract

Suggested is a process for obtaining immunoglobulins, wherein
(a) colostral milk from days 0 to 7 is subjected to thermal treatment, skimming the cream, (b) the skimmed milk such obtained is subjected to sterile microfiltration thus producing a first retentate R 1 which contains the casein, and a first permeate P 1 , (c) the first permeate P 1 is subjected to ultrafiltration thus producing a second permeate P2 which contains lactose and minerals, and a second retentate R 2 , in which the immunoglobulins are concentrated.

First claim

The invention claimed is:

1. A process for obtaining immunoglobulins consisting essentially of:
(a) colostral milk from days 0to 7is subjected to thermal treatment, skimming the cream,
(b) the skimmed milk such obtained is subjected at a temperature of from about 50 to about 70° C. to a sterile microfiltration thus producing a first retentate R 1 which contains the casein, and a first permeate P 1 ,
(c) the first permeate P 1 is subjected at a temperature of from about 5 to about 15° C. to ultrafiltration thus producing a second permeate P 2 which contains lactose and minerals, and a second retentate R 2 , in which the immunoglobulins are concentrated, and
(d) the immunoglobulins thus obtained from the second retentate are separated off from by treatment with anion exchanger.

2. The process of claim 1 wherein the colostral milk is treated at a temperature of from about 70 to about 80° C.

3. The process of claim 1 , wherein the colostral milk is treated for a period from about 10 to about 30 s.

4. The process of claim 1 , wherein microfiltration is performed using membranes having a selective separation of from about 0.1 to about 0.2 μm.

5. The process of claim 1 , wherein microfiltration is performed by means of a ceramic membrane.

6. The process of claim 1 , wherein ultrafiltration is performed using membranes having a selective separation of from about 0.01 to about 0.1 μm.

7. The process of claim 1 , wherein ultrafiltration is performed by means of a spiral coil membrane.

8. The process of claim 1 , wherein separation is performed by means of an anion exchanger at a temperature within the range of from about 2 to about 25° C.

9. The process of claim 1 , wherein separation is performed by means of an anion exchanger at a pH value of from about 6 to about 7.

Assignees

• Dmk Deutsches Milchkontor Gmbh

Inventors

• Döring Sven-Rainer

Classifications

• A23J3/08

  Dairy proteins · CPC title

• A23C9/1422

  by ultrafiltration, microfiltration or diafiltration of milk, e.g. for separating protein and lactose; Treatment of the UF permeate · CPC title

• C07K16/04 Primary

  from milk · CPC title

• A23C9/206

  Colostrum; Human milk · CPC title