Compositions comprising secretory IgA and probiotics

Patent metadata

Field	Value
Publication number	US-12350332-B2
Application number	US-202017597051-A
Country	US
Kind code	B2
Filing date	Jun 25, 2020
Priority date	Jun 28, 2019
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

The present invention relates to methods for producing compositions comprising secretory IgA and one or more probiotics. In particular, the invention relates to methods for producing compositions in which the secretory IgA and one or more probiotics are associated. The invention also relates to compositions obtainable by the methods and uses of the compositions, for example in reducing or preventing a non-viral infection and/or inflammation.

First claim

The invention claimed is:

1. A method for production of a composition comprising secretory IgA and a probiotic, the method comprising the steps of:
(a) providing a source of SIgA in the form of milk or a fraction thereof;
(b) pasteurizing the milk at a temperature of 82-84° C. for a duration of 5-7 seconds(s); and
(c) admixing with the probiotic, wherein the admixing is carried out at a temperature of 1-45° C. for a duration of 10-1440 minutes (min).

2. The method of claim 1 , wherein the admixing of step (c) is carried out at a temperature of 36-38° C. for a duration of 28-32 min.

3. The method of claim 1 , wherein the amount of secretory IgA per 2×10 7 CFU of the probiotic in the mixture of step (c) is 0.34 ng-20 μg.

4. The method of claim 1 , wherein the concentration of the probiotic in the mixture of step (c) is 1×10 6 -1×10 11 CFU/mL.

5. The method of claim 1 , wherein the milk provided in step (a) has not been exposed to a temperature of greater than 70° C., following expression.

6. The method of claim 1 , wherein the probiotic is Bifidobacterium lactis or Bifidobacterium longum subspecies infantis.

7. The method of claim 1 , wherein the secretory IgA and the probiotic are at least partially associated in the composition.

8. The method of claim 1 , wherein the composition comprises 0.0001-10 mg secretory IgA per daily dose.

9. The method of claim 1 , wherein the composition comprises between 1×10 2 and 1×10 12 cells of probiotics per daily dose.

Assignees

• Nestle Sa

Inventors

• Favre Laurent
• Hugelshofer Daniel

Classifications

• C07K16/04

  from milk · CPC title

• A61K35/745

  Bifidobacteria · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61P31/00

  Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics · CPC title

• A61K39/39508 Primary

  from milk, i.e. lactoglobulins · CPC title