Lactobacillus paracasei strain
US-12152232-B2 · Nov 26, 2024 · US
Lactobacillus plantarum cells with improved resistance to high concentrations of ethanol
US9469831B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9469831-B2 |
| Application number | US-201214125890-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 14, 2012 |
| Priority date | Jun 17, 2011 |
| Publication date | Oct 18, 2016 |
| Grant date | Oct 18, 2016 |
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Abstract
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Cycloserine resistant mutants of lactic acid bacteria characterized by having improved resistance towards ethanol. The cycloserine resistant mutants of lactic acid bacteria can e.g. be used for malolactic fermentations of wine (i.e. including sparkling wine such as Cava/champagne) having high alcohol levels.
First claim
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The invention claimed is: 1. A method for producing a wine, comprising administering to a grape juice or wine a Lactobacillus plantarum composition comprising from 10 4 to 10 14 CFU/g Lactobacillus plantarum cells, wherein the Lactobacillus plantarum cells: (i) have a resistance to D-cycloserine, such that the amount of D-cycloserine required to reduce the OD 600 measured growth of the Lactobacillus plantarum cells by 50% after 24 hours growth at 18° C. in Grape Juice GJ-5 medium as compared to growth in Grape Juice GJ-5 medium without added D-cycloserine is higher than 70 μg/ml D-cycloserine; and (ii) wherein the D-cycloserine resistant Lactobacillus plantarum cells have a resistance towards ethanol, such that the cells can grow to an OD 600 of at least 0.8 after 3 days incubation at 25° C. in Grape Juice GJ-5 medium with 11% ethanol. 2. The method of claim 1 , wherein the wine is selected from the group consisting of red wine, white wine and sparkling wine. 3. The method of claim 1 , wherein the administered composition comprises from 10 4 CFU to 10 8 CFU Lactobacillus plantarum cells per ml grape juice or wine. 4. The method of claim 1 , wherein the the Lactobacillus plantarum cells have a resistance to D-cycloserine, such that the amount of D-cycloserine required to reduce the OD 600 measured growth of the Lactobacillus plantarum cells by 50% after 24 hours growth at 18° C. in Grape Juice GJ-5 medium as compared to growth in Grape Juice GJ-5 medium without added D-cycloserine is higher than 80μg/ml D-cycloserine. 5. The method of claim 1 , wherein the the Lactobacillus plantarum cells have a resistance to D-cycloserine, such that the amount of D-cycloserine required to reduce the OD 600 measured growth of the Lactobacillus plantarum cells by 50% after 24 hours growth at 18° C. in Grape Juice GJ-5 medium as compared to growth in Grape Juice GJ-5 medium without added D-cycloserine is higher than 90 μg/ml D-cycloserine. 6. The method of claim 1 , wherein the Lactobacillus plantarum cells have a resistance towards ethanol, such that the cells can grow to an OD 600 of at least 0.8 after 3 days incubation at 25° C. in Grape Juice GJ-5 medium with 12% ethanol. 7. The method of claim 1 , wherein the Lactobacillus plantarum cells have a resistance towards ethanol, such that the cells can grow to an OD 600 of at least 0.8 after 3 days incubation at 25° C. in Grape Juice GJ-5 medium with 13% ethanol. 8. The method of claim 1 , wherein the Lactobacillus plantarum composition comprises spray-dried Lactobacillus plantarum cells. 9. The method of claim 1 , wherein the Lactobacillus plantarum composition comprises frozen Lactobacillus plantarum cells. 10. The method of claim 1 , wherein the administered composition comprises from 10 5 to 10 7 CFU Lactobacillus plantarum cells per ml grape juice or wine. 11. The method of claim 1 , wherein the administered composition comprises about 10 6 CFU Lactobacillus plantarum cells per ml grape juice or wine.
Assignees
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Classifications
Adaptation or attenuation of cells · CPC title
- C12N1/20Primary
Bacteria; Culture media therefor · CPC title
- C12G1/0203Primary
by microbiological or enzymatic treatment · CPC title
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Frequently asked questions
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- What does patent US9469831B2 cover?
- Cycloserine resistant mutants of lactic acid bacteria characterized by having improved resistance towards ethanol. The cycloserine resistant mutants of lactic acid bacteria can e.g. be used for malolactic fermentations of wine (i.e. including sparkling wine such as Cava/champagne) having high alcohol levels.
- Who is the assignee on this patent?
- Soerensen Kim Ib, Kibenich Annette, Johansen Eric, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N1/20. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 18 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).