Methods and Compositions for Generating Bioactive Assemblies of Increased Complexity and Uses
US-2015374846-A1 · Dec 31, 2015 · US
Manufacturing method for silica encapsulated single-enzyme nanoparticles and single-enzyme nanoparticles manufactured by means of method
US9458448B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9458448-B2 |
| Application number | US-201214112834-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 23, 2012 |
| Priority date | May 23, 2011 |
| Publication date | Oct 4, 2016 |
| Grant date | Oct 4, 2016 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
A method for manufacturing single enzyme nanoparticles through silica encapsulation according to the present disclosure does not include a surface functionalization process and a polymerization process during the synthesis, and reaction conditions are mild. Thus, the method is appropriate for a large scale production.
First claim
Opening claim text (preview).
The invention claimed is: 1. Silica encapsulated single-enzyme nanoparticles comprising a single enzyme and a silica network surrounding the single enzyme and having pores, wherein no covalent bond is formed between the single enzyme and the silica network, and the silica encapsulated single-enzyme nanoparticles have a Michaelis-Menten constant (K m ) value with a margin of error not greater than 20% as compared to that of free enzymes, and wherein the silica network has a thickness of 0.5-2.0 nm. 2. The silica encapsulated single-enzyme nanoparticles according to claim 1 , wherein the silica encapsulated single-enzyme nanoparticles have a Michaelis-Menten constant (K m ) value with a margin of error not greater than 10% as compared to that of free enzymes. 3. The silica encapsulated single-enzyme nanoparticles according to claim 1 , wherein the silica encapsulated single-enzyme nanoparticles have a Michaelis-Menten constant (K m ) value with a margin of error not greater than 3% as compared to that of free enzymes. 4. The silica encapsulated single-enzyme nanoparticles according to claim 1 , wherein the pore has a size of 0.1-2 nm.
Assignees
Inventors
Classifications
- C12N9/96Primary
Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title
Enzymes or microbial cells immobilised on or in an inorganic carrier · CPC title
Manufacture or treatment of nanostructures · CPC title
Nanoparticles · CPC title
Triglyceride splitting, e.g. by means of lipase · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9458448B2 cover?
- A method for manufacturing single enzyme nanoparticles through silica encapsulation according to the present disclosure does not include a surface functionalization process and a polymerization process during the synthesis, and reaction conditions are mild. Thus, the method is appropriate for a large scale production.
- Who is the assignee on this patent?
- Kim Jungbae, Hong Sung-Gil, Lee Byoungsoo, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N9/96. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 04 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).