Arrayed dual axis confocal microscopes

Patent metadata

Field	Value
Publication number	US-9448394-B2
Application number	US-201414209839-A
Country	US
Kind code	B2
Filing date	Mar 13, 2014
Priority date	Mar 14, 2013
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

Various aspects as described herein are directed to apparatuses, methods, and systems including a sandwiched arrangement having a light-access port, a scanning mirror, an optics region, and a spacer. The spacer provides a light-directing optical region that separates the scanning mirror and the optics region, and includes a mirrored surface that reflects light between the light-access port and the optics region. Additionally, the optics region includes a curved-shaped window that provides a field of view by communicating beams of light between a target region. The optics region also includes a curved-shaped mirror having a surface that reflects light between the scanning mirror and the mirrored surface. Light beams, as conveyed between the light-access port and the curved-shaped window, are folded by being reflected off the scanning mirror, the curved-shaped mirror and the mirrored surface.

First claim

The invention claimed is:

1. An apparatus comprising:
a sandwiched arrangement of a plurality of confocal microscopes that form an array, the arrangement including
a plurality of light-access ports,
a plurality of scanning mirrors,
a substrate with a pattern of optical components being replicated as part of the substrate to form an array of curved-shaped mirrors and windows, and
a spacer including a pattern of replicated cavities in the spacer, the replicated cavities configured and arranged to direct light between the plurality of light-access ports and the array of curved-shaped mirrors using inclined mirrored surfaces in the cavities, and to separate the scanning mirrors and the substrate;
wherein each confocal microscope in the plurality of confocal microscopes is configured and arranged to:
provide a field of view by communicating beams of light between a corresponding target region, located in a direction opposite a corresponding scanning mirror, and a corresponding light-directing optical region; and

fold light beams, as conveyed between a corresponding light-access port and a corresponding curved-shaped window, by reflecting the light beams off a corresponding scanning mirror, the corresponding curved-shaped mirror and a corresponding inclined mirrored surface.

2. The apparatus of claim 1 , wherein the windows are hemispherical windows, and the curved-shaped mirrors are ellipsoidal mirrors, and wherein the inclined mirrored surfaces in the spacer are oppositely situated near end regions of a replicated first pattern of the optics region.

3. The apparatus of claim 1 , further comprising a scanning mirror substrate that is bonded with the plurality of scanning mirrors.

4. The apparatus of claim 1 , further including a flex circuit configured to provide connections to the confocal microscopes, each connection configured to control movement of a corresponding scanning mirror of the plurality of scanning mirrors.

5. The apparatus of claim 1 , wherein the plurality of confocal microscopes are arranged in a single row forming a linear array and the spacer includes grooves for receiving a plurality of optical fibers that are connected to the plurality of light-access ports.

6. The apparatus of claim 1 , wherein the plurality of confocal microscopes are arranged in an X-by-Y array of confocal microscopes, wherein each of X and Y is two or greater.

7. The apparatus of claim 1 , wherein the sandwiched arrangement includes flex circuitry including connections to the plurality of scanning mirrors, the flex circuitry configured and arranged to control movement of the scanning mirrors during the capture of images.

8. The apparatus of claim 1 , wherein a surface of the substrate is configured and arranged to interface with a tissue region, and the sandwiched arrangement includes a plurality of optical fiber pairs, each optical fiber pair connected to the light-access ports of a respective confocal microscope of the plurality of confocal microscopes.

9. The apparatus of claim 8 , wherein each confocal microscope of the plurality of confocal microscopes is configured and arranged to capture images of the tissue region at a resolution between 1-5 μm and a field-of-view of at least 300 μm by 300 μm.

10. The apparatus of claim 9 , wherein the plurality of confocal microscopes are arranged in an array, that is at least 10-by-10, the sandwiched arrangement further includes a flex circuit that includes control wires that are configured to synchronously drive each of the scanning mirrors using the same driving voltages at each scanning mirror and the plurality of confocal microscopes are configured to capture a 3 centimeter by 3 centimeter image in 10 seconds.

11. The apparatus of claim 10 , wherein the plurality of optical fiber pairs are vertically coupled to the plurality of light-access ports from a side of the substrate opposite the surface of the substrate.

12. The apparatus of claim 1 , wherein the windows are tapered gradient-index (GRIN) lenses that each includes a tip configured and arranged to penetrate tissue, and to focus beams of light near the tip.

13. A method for use in a sandwiched arrangement of a plurality of confocal microscopes that form an array, the arrangement including a plurality of light-access ports, a plurality of scanning mirrors, and a substrate with a pattern of optical components being replicated as part of the substrate to form an array of curve-shaped mirrors and windows, the method comprising:
using a spacer, that includes a pattern of replicated cavities in the spacer, to separate the scanning mirrors and the substrate;
reflecting, using the plurality of curved-shaped mirrors, light between the light-access ports and the windows; and
for each confocal microscope and corresponding components, folding beams of light between a light access port of the light access ports and a target region by:
using a window of the windows to provide a field of view by communicating beams of light between a scanning mirror of the scanning mirrors and the target region, located in a direction opposite the scanning mirror; and
using a curved-shaped mirror of the curved-shaped mirrors to reflect light between the scanning mirror and a mirrored surface of the mirrored surfaces.

14. The method of claim 13 , further including using the plurality of confocal microscopes to inspect a target area via respective fields of view provided by the plurality of confocal microscopes.

15. The method of claim 14 , further comprising driving scanning mirrors for individual rows of the confocal microscopes using parallel connections within each of the individual rows.

16. The method of claim 13 , wherein the windows are gradient index (GRIN) lenses.

17. The method of claim 16 , wherein the GRIN lenses are tapered and further comprising placing different allergens on the GRIN lenses, puncturing tissue with the tapered GRIN lenses and detecting inflammatory cells from images captured by the plurality of confocal microscopes.

Assignees

• Univ Leland Stanford Junior

Inventors

• Mandella Michael J
• Solgaard Olav
• Contag Christopher H

Classifications

• G02B21/0048 Primary

  scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors · CPC title

• G02B21/008

  Details of detection or image processing, including general computer control · CPC title

• A61B5/0071

  by measuring fluorescence emission · CPC title

• A61B5/0068

  Confocal scanning · CPC title