Soybean ATPS promoter and its use in constitutive expression of transgenic genes in plants

Patent metadata

Field	Value
Publication number	US-9447424-B2
Application number	US-201214344326-A
Country	US
Kind code	B2
Filing date	Sep 13, 2012
Priority date	Sep 13, 2011
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean ATP sulfurylase (ATPS) and fragments thereof and theft use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

First claim

What is claimed is:

1. A recombinant DNA construct comprising:
a nucleotide sequence comprising a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5,
operably linked to at least one heterologous nucleotide sequence, wherein said nucleotide sequence is a promoter.

2. The recombinant DNA construct of claim 1 , wherein said promoter is a constitutive promoter.

3. A vector comprising the recombinant DNA construct of claim 1 .

4. A cell comprising the recombinant DNA construct of claim 1 .

5. The cell of claim 4 , wherein the cell is a plant cell.

6. A transgenic plant having stably incorporated into its genome the recombinant DNA construct of claim 1 .

7. The transgenic plant of claim 6 wherein said plant is a dicot plant.

8. The transgenic plant of claim 7 wherein the plant is soybean.

9. A transgenic seed produced by the transgenic plant of claim 6 , wherein the transgenic seed comprises the recombinant DNA construct.

10. The recombinant DNA construct according to claim 1 , wherein the heterologous nucleotide sequence is a sequence selected from the group consisting of: a reporter sequence, a selection marker, a disease resistance conferring sequence, a herbicide resistance conferring sequence, an insect resistance conferring sequence; a sequence that regulates carbohydrate metabolism, a sequence that regulates fatty acid metabolism, a sequence that regulates amino acid metabolism, a sequence that regulates drought resistance, a sequence that regulates cold resistance, a sequence that regulates heat resistance and a sequence that regulates salt resistance in plants.

11. A plant stably transformed with a recombinant DNA construct comprising a soybean embryo-specific promoter and a heterologous nucleotide sequence operably linked to said embryo-specific promoter, wherein said embryo-specific promoter controls expression of said heterologous nucleotide sequence in a plant cell, and further wherein said embryo-specific promoter comprises a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5.

Assignees

• Li Zhongsen
• Du Pont

Inventors

• Li Zhongsen

Classifications

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/158

  Expression markers · CPC title

• C12N15/8245

  involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis · CPC title