Short multi-repeat rna targeting gene silencing
US-2024182899-A1 · Jun 6, 2024 · US
Compositions and methods for increased expression in sugar cane
US9441234B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9441234-B2 |
| Application number | US-201214364184-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 7, 2012 |
| Priority date | Dec 15, 2011 |
| Publication date | Sep 13, 2016 |
| Grant date | Sep 13, 2016 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
A sugar cane plant cell comprising a recombinant nucleic acid molecule is provided, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotide sequence of interest, and the nucleotide sequence of interest is expressed at a level at least about 6 times greater than the level of expression of said nucleotide sequence of interest in a control. Additionally, a method of increasing the expression of a nucleotide sequence of interest in a sugar cane plant cell using the recombinant nucleic acid molecule of the invention is provided.
First claim
Opening claim text (preview).
That which is claimed: 1. A sugar cane plant cell comprising a recombinant nucleic acid molecule, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotide sequence of interest, and the nucleotide sequence of interest is expressed at a level at least about 6 times greater than the level of expression of said nucleotide sequence of interest in a control, further wherein the promoter is a maize polyubiquitin-1 promoter and/or a maize PepC promoter. 2. The sugar cane plant cell of claim 1 , wherein the recombinant nucleic acid molecule further comprises a Kozak sequence. 3. A sugar cane plant or sugar cane plant part comprising the plant cell of claim 1 . 4. A crop comprising a plurality of the sugar cane plants of claim 3 planted together in an agricultural field. 5. A method of increasing the expression of a nucleotide sequence of interest in a sugar cane plant cell, comprising: introducing into the sugar cane plant cell a recombinant nucleic acid molecule, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotide sequence of interest, under conditions where the nucleotide sequence of interest is expressed, further wherein the promoter is a maize polyubiquitin-1 promoter and/or a maize PepC promoter, thereby increasing the level of expression of the nucleotide sequence of interest to at least about 6 times greater than the level of expression of said nucleotide sequence of interest in a control. 6. The method of claim 5 , wherein the recombinant nucleic acid molecule further comprises a Kozak sequence. 7. The method of claim 5 , further comprising regenerating a plant from the plant cell. 8. A sugar cane plant cell produced by the method of claim 4 . 9. A sugar cane plant produced by the method of claim 7 . 10. A crop comprising a plurality of the sugar cane plants of claim 9 planted together in an agricultural field. 11. The method of claim 6 , further comprising regenerating a plant from the plant cell. 12. A sugar cane plant cell produced by the method of claim 5 .
Assignees
Inventors
Classifications
- C12N15/8216Primary
Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title
- C12N15/8245Primary
involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9441234B2 cover?
- A sugar cane plant cell comprising a recombinant nucleic acid molecule is provided, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotid…
- Who is the assignee on this patent?
- Kinkema Mark D, Miles Stacy, Syngenta Participations Ag, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/8216. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 13 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).