Estimating molecular weight of hydrocarbons
US-12140585-B2 · Nov 12, 2024 · US
Chromatography media and use thereof
US9433923B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9433923-B2 |
| Application number | US-201314407892-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 11, 2013 |
| Priority date | Jun 14, 2012 |
| Publication date | Sep 6, 2016 |
| Grant date | Sep 6, 2016 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for isolating proteins from a sample comprising: a. combining a sample comprising glutathione-S-transferase (GST) tagged proteins with an affinity chromatography media comprising non-crosslinked agarose coated magnetic beads having glutathione immobilized thereon; and b. magnetically separating the GST tagged proteins bound to the affinity media from the sample, thereby isolating the proteins from the sample. 2. The method of claim 1 , wherein the GST tagged proteins are combined directly after cell culture to the chromatography media. 3. The method of claim 2 , wherein the GST tagged proteins are concentrated after cell culture before combining with the chromatography media. 4. The method of claim 1 , wherein the affinity chromatography media provides improved isolation of GST tagged proteins over an affinity chromatography media produced from crosslinked agarose coated magnetic beads having glutathione immobilized thereon.
Assignees
Inventors
Classifications
Magnetic properties · CPC title
- B01J20/291Primary
Gel sorbents · CPC title
containing a GST-tag · CPC title
Affinity chromatography · CPC title
Proteins, nucleic acids, polysaccharides, antibodies or antigens · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9433923B2 cover?
- The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins.
- Who is the assignee on this patent?
- Ge Healthcare Bio Sciences Ab
- What technology area does this patent fall under?
- Primary CPC classification B01J20/291. Mapped technology areas include Operations & Transport.
- When was this patent published?
- Publication date Tue Sep 06 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).