Chromatographic medium

Patent metadata

Field	Value
Publication number	US-9726650-B2
Application number	US-201314375677-A
Country	US
Kind code	B2
Filing date	Feb 1, 2013
Priority date	Feb 3, 2012
Publication date	Aug 8, 2017
Grant date	Aug 8, 2017

Abstract

A chromatographic medium having a separating agent layer, which is used to separate target substances, a filling agent layer, which is used to fix the target substances before the target substances are separated, and a permeation layer, which is used to enable permeation of the target substances separated by the separating agent layer, wherein the filling agent layer comes into contact with the separating agent layer via a plane that intersects the direction of development of the target substances in the chromatographic medium and is positioned on the upstream side in the direction of development, the separating agent layer exhibits separability of the target substances and optical responsiveness to ultraviolet rays, and the permeation layer exhibits an optical responsiveness that is different from those of the target substances and the separating agent layer.

First claim

The invention claimed is:

1. A chromatographic medium comprising:
a separating agent layer, which is used to separate target substances;
a filling agent layer, which is used to fix the target substances before the target substances are separated; and
a permeation layer, which is disposed in a different plane from the separating agent layer, used to enable permeation of the target substances separated by the separating agent layer and contains a porous material and a fluorescent indicator or coloring reagent as constituent materials,
wherein the permeation layer is laminated to a region of a surface of the separating agent layer or to a region of a surface of the filling agent layer by applying a slurry containing the porous material and the fluorescent indicator or coloring reagent and then drying the slurry and not laminated to another region of the surface of the separating agent layer or another region of the surface of the filling agent layer, the filling agent layer comes into contact with the separating agent layer at a plane that is approximately perpendicular to a direction of development of the target substances in the chromatographic medium and is positioned on an upstream side in the direction of development,
the separating agent layer exhibits a separability of the target substances and exhibits an optical responsiveness to ultraviolet rays, and
the permeation layer exhibits an optical responsiveness that is different from those of the target substances and the separating agent layer.

2. The chromatographic medium according to claim 1 , wherein the filling agent layer is laminated in a region of the chromatographic medium extending from a bottom edge of the chromatographic medium and having a length which is 1/20 to 1/2 of the length of the chromatographic medium in the direction of development.

3. The chromatographic medium according to claim 1 , wherein the permeation layer is laminated in the form of dots on the separating agent layer.

4. The chromatographic medium according to claim 3 , wherein in the permeation layer laminated in the form of dots, the average diameter of the dots is 0.01 to 5 mm and the pitch between dots is 0.015 to 5 mm.

5. The chromatographic medium according to claim 1 , wherein the permeation layer is laminated on the separating agent layer as band-like rows that intersect a direction of development of the chromatographic medium.

6. The chromatographic medium according to claim 5 , wherein bands that form the band-like rows are selected from among straight lines, wavy lines and dashed lines thereof.

7. The chromatographic medium according to claim 1 , wherein the permeation layer is thinner in depth than the separating agent layer.

8. The chromatographic medium according to claim 1 , wherein a separating agent that constitutes the separating agent layer is a separating agent for optical isomers.

9. The chromatographic medium according to claim 8 , wherein the separating agent for optical isomers contains a polysaccharide derivative formed of a polysaccharide and one type of group selected from the group consisting of aromatic ester groups, aromatic carbamoyl groups, aromatic ether groups and carbonyl groups that replace some or all of hydroxyl groups or amino groups in the polysaccharide.

10. The chromatographic medium according to claim 1 , wherein the filling agent layer contains a porous material as a constituent material.

11. The chromatographic medium according to claim 10 , wherein the permeation layer is laminated in a discontinuous manner in the direction of development of the chromatographic medium.

12. The chromatographic medium according to claim 1 , wherein the porous material is silica gel or surface-treated silica gel.

13. The chromatographic medium according to claim 1 , wherein the permeation layer further comprises a binder as a constituent material.

14. The chromatographic medium according to claim 1 , wherein scale marks and/or characters are present on the permeation layer.

15. The chromatographic medium according to claim 14 , wherein the scale marks and/or characters exhibit optical responsiveness that are different from those of the permeation layer.

16. The chromatographic medium according to claim 1 , additionally comprising a base material which faces the separating agent layer or the permeation layer.

17. The chromatographic medium according to claim 1 , wherein the chromatographic medium is plate-shaped, cylindrical or columnar.

18. A TLC plate comprising:
the chromatographic medium according to claim 1 ; and
a base material used to support the chromatographic medium,
wherein the chromatographic medium is laminated on a plurality of regions on the base material.

19. A TLC material comprising:
the chromatographic medium according to claim 1 ; and
a base material used to support the chromatographic medium.

Assignees

• Daicel Corp

Inventors

• Minoda Toshiharu
• Ikeda Isamu

Classifications

• B01J2220/46

  Materials comprising a mixture of inorganic and organic materials (materials coated or impregnated on a carrier B01J20/32) · CPC title

• G01N30/92 Primary

  Construction of the plate · CPC title

• B01D15/08

  Selective adsorption, e.g. chromatography · CPC title

• G01N30/93

  Application of the sorbent layer · CPC title

• G01N2030/945

  Application of reagents to undeveloped plate · CPC title