Cells or plants producing polylactate or its copolymers and uses thereof

Patent metadata

Field	Value
Publication number	US-9410167-B2
Application number	US-43951706-A
Country	US
Kind code	B2
Filing date	May 24, 2006
Priority date	May 24, 2005
Publication date	Aug 9, 2016
Grant date	Aug 9, 2016

Abstract

The present invention relates to cells or plants that can produce polylactate or its copolymers and to a method for preparing polylactate or its copolymers using the same. More specifically, cells or plants with the ability to produce polylactate or hydroxyalkanoate-lactate copolymers comprise both a gene encoding an enzyme that converts lactate into lactyl-CoA and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses lactyl-CoA as a substrate. Also described is a method for preparing polylactate or hydroxyalkanoate-lactate copolymers which comprises culturing the cells in a medium containing lactate or lactate and various hydroxyalkanoates or culturing the plants. Effective preparation of hydroxyalkanoate-lactate copolymer which comprises various hydroxyalkanoates as well as polylactate, using the cells or the plants, is disclosed.

First claim

What is claimed is:

1. A method for preparing polylactate or hydroxyalkanoate-lactate copolymer [poly(hydroxyalkanoate-co-lactate)], comprising:
culturing a recombinant E. coli or Ralstonia eutropha in a medium containing lactate, or lactate and hydroxyalkanoates as a carbon source, wherein the recombinant E. coli or Ralstonia eutropha comprises a propionyl-CoA tranferase gene (pct) derived from Clostridium propionicum , and a gene selected from phaC derived from Pseudomonas sp. 61-3 and phaRBC derived from Bacillus cereus ; and
recovering polylactate or hydroxyalkanoate-lactate copolymer from the cultured E. coli or Ralstonia eutropha.

2. The method according to claim 1 , wherein said hydroxy-alkanoate-lactate copolymer is one or more hydroxyalkanoates selected from the group consisting of 3-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, a C 6-14 (D)-3-hydroxycarboxylic acid, 3-hydroxypropionic acid, 3-hydroxyhexanoic acid, 3-hydroxyheptanoic acid, 3-hydroxyoctanoic acid, 3-hydroxynonanoic acid, 3-hydroxydecanoic acid, 3-hydroxyundecanoic acid, 3-hydroxydodecanoic acid, 3-hydroxytetradecanoic acid, 3-hydroxyhexadecanoic acid, 4-hydroxyvaleric acid, 4-hydroxyhexanoic acid, 4-hydroxyheptanoic acid, 4-hydroxyoctanoic acid, 4-hydroxydecanoic acid, 5-hydroxyvaleric acid, 5-hydroxy-hexanoic acid, 6-hydroxydodecanoic acid, 3-hydroxy-4-pentenoic acid, 3-hydroxy-4-trans-hexenoic acid, 3-hydroxy-4-cis-hexenoic acid, 3-hydroxy-5-hexenoic acid, 3-hydroxy-6-trans-octenoic acid, 3-hydroxy-6-cis-octenoic acid, 3-hydroxy-7-octenoic acid, 3-hydroxy-8-nonenoic acid, 3-hydroxy-9-decenoic acid, 3-hydroxy-5-cis-dodecenoic acid, 3-hydroxy-6-cis-dodecenoic acid, 3-hydroxy-5-cis-tetradecenoic acid, 3-hydroxy-7-cis-tetradecenoic acid, 3-hydroxy-5,8-cis-cis-tetradecenoic acid, 3-hydroxy-4-methylvaleric acid, 3-hydroxy-4-methylhexanoic acid, 3-hydroxy-5-methylhexanoic acid, 3-hydroxy-6-methylheptanoic acid, 3-hydroxy-4-methyloctanoic acid, 3-hydroxy-5-methyloctanoic acid, 3-hydroxy-6-methyloctanoic acid, 3-hydroxy-7-methyloctanoic acid, 3-hydroxy-6-methylnonanoic acid, 3-hydroxy-7-methylnonanoic acid, 3-hydroxy-8-methylnonanoic acid, 3-hydroxy-7-methyldecanoic acid, 3-hydroxy-9-methyl-decanoic acid, 3-hydroxy-7-methyl-6-octenoic acid, malic acid, 3-hydroxysuccinic acid-methyl-ester, 3-hydroxyadipinic acid-methylester, 3-hydroxysuberic acid-methylester, 3-hydroxyazelaic acid-methylester, 3-hydroxysebacic acid-methylester, 3-hydroxyazelaic acid-methyl-ester, 3-hydroxysuberic acid-ethylester, 3-hydroxysebacic acid-ethylester, 3-hydroxypimelic acid-propylester, 3-hydroxysebacic acid-benzylester, 3-hydroxy-8-acetoxyoctanoic acid, 3-hydroxy-9-acetoxynonanoic acid, phenoxy-3-hydroxybutyric acid, phenoxy-3-hydroxyvaleric acid, phenoxy-3-hydroxyheptanoic acid, phenoxy-3-hydroxyoctanoic acid, para-cyanophenoxy-3-hydroxybutyric acid, para-cyanophenoxy-3-hydroxyvaleric acid, para-cyanophenoxy-3-hydroxyhexanoic acid, para-nitrophenoxy-3-hydroxyhexanoic acid, 3-hydroxy-5-phenylvaleric acid, 3-hydroxy-5-cyclohexylbutyric acid, 3,12-dihydroxydodecanoic acid, 3,8-dihydroxy-5-cis-tetradecenoic acid, 3-hydroxy-4,5-epoxydecanoic acid, 3-hydroxy-6,7-epoxydodecanoic acid, 3-hydroxy-8,9-epoxy-5,6-cis-tetradecanoic acid, 7-cyano-3-hydroxyheptanoic acid, 9-cyano-3-hydroxynonanoic acid, 3-hydroxy-7-fluoroheptanoic acid, 3-hydroxy-9-fluorononanoic acid, 3-hydroxy-6-chlorohexanoic acid, 3-hydroxy-8-chlorooctanoic acid, 3-hydroxy-6-bromohexanoic acid, 3-hydroxy-8-bromooctanoic acid, 3-hydroxy-11-bromoundecanoic acid, 3-hydroxy-2-butenoic acid, 6-hydroxy-3-dodecenoic acid, 3-hydroxy-2-methylbutyric acid, 3-hydroxy-2-methylvaleric acid and 3-hydroxy-2,6-dimethyl-5-heptenoic acid.

3. The method according to claim 2 , wherein said hydroxyalkanoate-lactate copolymer is selected from the group consisting of poly(3HB-co-lactate), poly(4HB-co-lactate), poly(3HP-co-lactate), poly(3HB-co-4HB-co-lactate) and poly(3HP-co-4HB-co-lactate).

4. A method for preparing a copolymer of 3HA having three to twelve carbons and lactate [poly(3HA-co-lactate)], the method comprising:
culturing a recombinant E. coli or Ralstonia eutropha in a medium containing lactate and a fatty acid as a carbon source, wherein the recombinant E. coli or Ralstonia eutropha comprises a propionyl-CoA transferase gene (pct) derived from Clostridium propionicum and a gene selected from phaC derived from Pseudomonas sp. 61-3 and phaRBC derived from Bacillus cereus ; and
recovering said [poly(3HA-co-lactate)] from the cultured E. coli or Ralstonia eutropha.

5. The method according to claim 4 , wherein the copolymer is [poly(3HHx-co-3HO-co-3HD-co-lactate)], and said fatty acid is decenoic acid and said 3HA is 3HHx, 3HO and 3HD.

6. The method according to claim 4 , wherein the copolymer is [poly(3HHx-co-3HO-co-3HD-co-3HDD-lactate)], and said fatty acid is dodecenoic acid and said 3HA is 3HHx, 3HO, 3HD and 3HDD.

Assignees

• Cho Jun-Hyeong
• Park Si-Jae
• Lee Sang-Yup
• Jung Yu-Kyung
• Lg Chemical Ltd
• Korea Advanced Inst Sci & Tech

Inventors

• Cho Jun-Hyeong
• Park Si-Jae
• Lee Sang-Yup
• Jung Yu-Kyung

Classifications

• C12P7/625 Primary

  Polyesters of hydroxy carboxylic acids · CPC title

• C12N2500/35

  Polyols, e.g. glycerin, inositol · CPC title

• C12N15/8243

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• C12N15/8242

  with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits · CPC title

• C12N15/74

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title