Pseudomonas putida strain as well as its microbial inoculum and application

Patent metadata

Field	Value
Publication number	US-9404163-B2
Application number	US-201514591740-A
Country	US
Kind code	B2
Filing date	Jan 7, 2015
Priority date	Sep 18, 2014
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

A Pseudomonas putida strain is named as Pseudomonas putida S-1, which was preserved at China Center for Type Culture Collection (CCTCC) on Sep. 25, 2013 with the deposit number of CCTCC NO: M2013444. The strain of this invention takes isopropanol, ethanal, dipropyl disulfide, diethyl disulfide and propanethiol as the sole carbon source for effective degradation of substrate simultaneously with the energy growth. Furthermore, it can obtain a perfect growth in different cultivation modes owing to high substrate tolerance, which has laid down a solid foundation for engineering application for elimination of volatile organic compounds contained exhaust gas by means of biological purification.

First claim

The invention claimed is:

1. A Pseudomonas putida strain characterized in that it is named as Pseudomonas putida S-1 and preserved on Sep. 25, 2013 with the number of CCTCC NO: M2013444.

2. The Pseudomonas putida strain as claimed in claim 1 wherein the Pseudomonas putida strain's cell is rod-shaped, with flagella but no gemmae; a small round bacteria colony is white with plump profile, smooth, wet and easy to be picked; the lawn grows along the streak, aerobic, with positive oxydase reaction; positive arginine hydrolase and catalase, and negative in indole reaction, methyl red reaction, Voges-Proskauer reaction and gram staining.

3. A microbial inoculum containing the Pseudomonas putida strain as claimed in claim 1 .

4. The microbial inoculum as claimed in claim 3 characterized in that it is in solid form.

5. The microbial inoculum as claimed in claim 4 characterized in that it is mixed by a liquid bacteria and a carrier; the carrier comprises an activated carbon powder, sawdust, dry soil and diatomaceous earth.

6. The microbial inoculum as claimed in claim 4 characterized in that the solid microbial inoculum is directly obtained through solid-state fermentation.

7. The microbial inoculum as claimed in claim 3 characterized in that it belongs to semi-solid microbial inoculum.

8. The microbial inoculum as claimed in claim 7 characterized in that the semi-solid microbial inoculum is obtained through semi-solid fermentation whose culture medium for semi-solid fermentation takes agar as the solid.

9. A method of decomposition of a volatile organic compound comprising the step of:
contacting the volatile organic compound with a Pseudomonas putida strain and a microbial inoculum containing the Pseudomonas putida strain,
wherein the Pseudomonas putida strain is named as Pseudomonas putida S-1 and preserved on Sep. 25, 2013 with the number of CCTCC NO: M2013444; and
wherein the volatile organic compound comprises isopropanol, ethanal, diethyl disulfide and propanethiol.

10. The method as claimed in claim 9 characterized in that the decomposition is conducted at a pH value of 4.0˜10.0, a temperature of 15° C.˜370° C. and salinity of 0%˜3%.

11. A method for treating an exhaust gas containing a volatile organic compound comprising the following steps:
(1) transplanting Pseudomonas putida S-1 or a microbial inoculum containing such strain to a bioreactor;
(2) passing the exhaust gas through the bioreactor;
wherein the Pseudomonas putida strain is named as Pseudomonas putida S-1 and preserved on Sep. 25, 2013 with the number of CCTCC NO: M2013444; and
wherein the volatile organic compound comprises isopropanol, ethanal, diethyl disulfide and propanethiol.

12. The method as claimed in claim 11 characterized in that the bioreactor is used at a pH value of 6.0˜8.0, a temperature of 25° C.˜30° C. and salinity of 0%˜1.5%.

13. The method as claimed in claim 11 characterized in that the bioreactor is a stirred bioreactor, an airlift bioreactor or a biotrickling filter.

14. The method as claimed in claim 11 characterized in that the bioreactor is a biotrickling filter.

15. The method as claimed in claim 9 wherein the Pseudomonas putida strain's cell is rod-shaped, with flagella but no gemmae; a small round bacteria colony is white with plump profile, smooth, wet and easy to be picked; the lawn grows along the streak, aerobic, with positive oxydase reaction; positive arginine hydrolase and catalase, and negative in indole reaction, methyl red reaction, Voges-Proskauer reaction and gram staining.

16. The method as claimed in claim 10 wherein the Pseudomonas putida strain's cell is rod-shaped, with flagella but no gemmae; a small round bacteria colony is white with plump profile, smooth, wet and easy to be picked; the lawn grows along the streak, aerobic, with positive oxydase reaction; positive arginine hydrolase and catalase, and negative in indole reaction, methyl red reaction, Voges-Proskauer reaction and gram staining.

Assignees

• Univ Zhejiang Technology
• Zhejiang Univ Of Technolgy

Inventors

• Chen Jianmeng
• Chen Dongzhi
• Ye Jiexu
• Han Limei
• Sun Yiming

Classifications

• B01D53/84

  Biological processes · CPC title

• B01D2251/95

  Specific microorganisms · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• B01D2257/708

  Volatile organic compounds V.O.C.'s · CPC title

• C12R1/40 Primary

  Chemistry & Metallurgy · mapped topic