Insecticidal proteins and methods for their use

Patent metadata

Field	Value
Publication number	US-10006045-B2
Application number	US-201414912356-A
Country	US
Kind code	B2
Filing date	Aug 14, 2014
Priority date	Aug 16, 2013
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed:

1. A DNA construct comprising a nucleic acid molecule encoding an PIP-47 polypeptide, having insecticidal activity, operably linked to a heterologous regulatory element, wherein the encoded PIP-47 polypeptide comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 2.

2. The DNA construct of claim 1 , wherein the encoded PIP-47 polypeptide comprises 1 to 60 amino acid substitutions compared to the native amino acid at position 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 57, 58, 62, 63, 65, 66, 67, 68, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 78, 79, 80, 81, 84, 85, 86, 88, 90, 91, 92, 95, 96, 97, 98, 99, 100, 103, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 107, 109, 110, 112, 115, 116, 117, 119, 120, 121, 124, 130, 132, 133, 135, 136, 137, 141, 142, 143, 144, 146, 147, 148, 149, 150, 151, 153, 156, 158, 161, 164, 165, 168, 169, 170, 172, 173, 174, 176, 178, 179, 181, 182, 184, 185, 188, 189, 193, 194, 196, 198, 200, 202, 203, 205, 206, 207, 208, 212, 213, 221, 222, 226, 228, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 250, 253, 254, 255, 257, 260, 262, 264, 265, 267, 270, 272, 273, 274, 275, 276, 277, 279, 280, 281, 282, 283, 284, 287, and 295 of SEQ ID NO: 2, in any combination, and optionally the encoded PIP-47 polypeptide further comprises a deletion of 1 to 5 amino acids, an insertion of 1 to 5 amino acids, addition of one or more amino acids at the N-terminus or addition of one or more amino acids at the C-terminus, in any combination compared to SEQ ID NO: 2.

3. The DNA construct of claim 1 , wherein the encoded PIP-47 polypeptide comprises 1 to 60 amino acid substitutions compared to the native amino acid at position 2, 4, 5, 6, 7, 9, 10, 13, 19, 20, 21, 22, 24, 25, 26, 29, 30, 37, 38, 41, 42, 43, 46, 47, 49, 50, 53, 62, 66, 68, 71, 73, 75, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 95, 97, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 136, 142, 161, 170, 172, 173, 174, 181, 205, 212, 213, 221, 230, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 257, 260, 262, 264, 272, 276, 279, 281, 283, 284, 287 and 295 of SEQ ID NO: 2, in any combination, and optionally the encoded PIP-47 polypeptide further comprises a deletion of 1 to 5 amino acids, an insertion of 1 to 5 amino acids, addition of one or more amino acids at the N-terminus or addition of one or more amino acids at the C-terminus, in any combination compared to SEQ ID NO: 2.

4. The DNA construct of claim 1 or 3 , wherein the encoded PIP-47 polypeptide comprises an amino acid sequence of the formula

(SEQ ID NO: 481)

Met His Ala Pro Gly Ala Ile Pro Ser Glu Lys Glu

1               5                   10

Ser Ala His Ala Trp Leu Thr Glu Thr Lys Ala Asn

        15                  20

Ala Lys Ser Thr Ala Leu Arg Gly Asn Ile Phe Ala

25                  30                  35

Gln Asp Tyr Asn Arg Gln Leu Leu Thr Ala Xaa Gly

            40                  45

Gln Ser Met Arg Ser Gly Ala Asp Ala Ile Asn Pro

    50                  55                  60

Phe Phe Ser Pro Ala Lys Gly Thr Ala Thr Gly Ser

                65                  70

Xaa Ala Lys Asp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

        75                  80

Xaa Xaa Pro Val Ser Ile Tyr Glu Gly Leu Xaa Thr

85                  90                  95

Ala Ile Asp Ile Ala Arg Arg Arg Ser Gly Xaa Xaa

            100                 105

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

    110                 115                 120

Ala Gly Asp Arg Glu His Phe Ile Ala Phe Thr Gln

                125                 130

Gln Ile Ala Glu Ile Pro Phe Leu Ser Xaa Leu Ala

        135                 140

Ala Gln Val Thr Gln Ile Gln Gln Lys Ser His Asp

145                 150                 155

Ala Asn Ala Leu Val Asp Ser Phe Val Lys Gly Phe

            160                 165

Ile Xaa Leu Lys Asn Gln Asp Val Glu Gln Ile Lys

    170                 175                 180

Gln Ser Leu Ser Ser Leu Val Asn Ala Ala Leu Ser

                185                 190

Tyr Ser Glu Gln Thr Glu Arg Gln Ser Asn Phe Asn

        195                 200

Xaa Asn Ile Leu Gln Thr Gly Asp Ser Gly Ser Val

205                 210                 215

Asn Phe Met Leu Xaa Ala Ser Glu Phe Thr Ile Lys

            220                 225

Ala Ser Ser His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa

    230                 235                 240

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ile Tyr Gln Leu Ser

                245                 250

Val Glu Ser Trp Asn Asn Val Lys Asp Val Phe Ser

        255                 260

Lys Gln Gln Lys Thr Asp Thr Gln Gln Trp Leu Gly

265                 270                 275

Asp Thr Xaa Thr Gln Val Arg Glu Gly Ser Lys Leu

            280                 285

Arg Ala Ile Cys Leu Val Ser

Assignees

• Pioneer Hi Bred Int
• Du Pont
• Pioneer Hi Bred Int
• Du Pont

Inventors

• Liu Lu
• O'Rear Jessica
• Park Young Jun
• Rosen Barbara
• Schellenberger Ute
• Schepers Eric
• Wei Jun-Zhi
• Xie Weiping
• Yalpani Nasser
• Zhong Xiaohong
• Zhu Genhai

Classifications

• C07K14/21 Primary

  from Pseudomonadaceae (F) · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• A01N63/02

  Human Necessities · mapped topic