Lipase variants and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9394530-B2
Application number	US-201314372858-A
Country	US
Kind code	B2
Filing date	Jan 25, 2013
Priority date	Feb 3, 2012
Publication date	Jul 19, 2016
Grant date	Jul 19, 2016

Abstract

The present invention relates to lipase variants and methods of obtaining them. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

First claim

The invention claimed is:

1. A lipase variant, comprising a substitution G91Q corresponding to the position of the mature polypeptide of SEQ ID NO: 2, wherein the variant has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2, and has lipase activity.

2. The variant of claim 1 , which has at least 95% sequence identity to the amino acid sequence of the parent lipase.

3. The variant of claim 1 , which has at least 97% sequence identity to the amino acid sequence of the parent lipase.

4. The variant of claim 1 , which has at least 98% sequence identity to the amino acid sequence of the parent lipase.

5. The variant of claim 1 , which further comprises a substitution at one or more positions corresponding to positions D96, T143, A150, E210, G225, T231, N233 and P250.

6. The variant of claim 5 , wherein the substitution comprises one or more of D96G, T143A, A150G, E210Q, G225R, T231R, N233R and P250R.

7. The variant of claim 1 , wherein said variant comprises the following substitutions:
a) G91Q +T143A +E210Q +T231R +N233R +P250R;
b) G91Q +A150G +E210Q +T231R +N233R +P250R; or
c) G91Q +E210Q +T231R +N233R +P250R.

8. The variant of claim 1 , which in comparison with the parent lipase has improved performance in the presence of an organic catalyst selected from the group consisting of organic catalysts having the following formulae:
a)

b)

or
c) mixtures thereof,

wherein each R1 is independently a branched alkyl group containing from 3 to 24 carbons or a linear alkyl group containing from 1 to 24 carbons; and recovering the variant.

9. The variant of claim 8 , wherein R1 is independently selected from the group consisting of 2-propylheptyl, 2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, n-dodecyl, n-tetradecyl, n-hexadecyl, n-octadecyl, iso-nonyl, iso-decyl, iso-tridecyl, and iso-pantadecyl.

Assignees

• Novozymes As

Inventors

• Erlandsen Luise
• Hansen Carsten Hoerslev
• Vind Jesper
• Svendsen Allan
• Sonksen Carsten Peter

Classifications

• C11D3/38627

  containing lipase · CPC title

• C12N9/20 Primary

  Triglyceride splitting, e.g. by means of lipase · CPC title

• C12N9/18

  Carboxylic ester hydrolases {(3.1.1)} · CPC title

• C12Y301/01

  Carboxylic ester hydrolases (3.1.1) · CPC title

• C11D3/392

  Heterocyclic compounds, e.g. cyclic imides or lactames · CPC title