Latex particles for particle agglutination assay

Patent metadata

Field	Value
Publication number	US-9383356-B2
Application number	US-201314431555-A
Country	US
Kind code	B2
Filing date	Sep 27, 2013
Priority date	Sep 27, 2012
Publication date	Jul 5, 2016
Grant date	Jul 5, 2016

Abstract

A particulate latex for high-sensitive agglutination assay that barely poses non-specific reactions and can readily prepare diagnostic reagents, and a reagent for agglutination assay including the particle are provided. A particulate latex for agglutination assay, including a first polymerizable monomer having a phenyl group, a second polymerizable monomer having a phenyl group and a salt of sulfonic acid, and a third polymerizable monomer represented by Formula (1):

CH 2 ═CR 1 —COOCH 2 CH 2 O(PO 2 )OCH 2 CH 2 —N(CH 3 ) 3   (1)

where R 1 represents a hydrogen atom or a methyl group,
wherein the density of functional groups in the third polymerizable monomer represented by Formula (1) on the surface of the particulate latex is 0.003 to 0.05 μmol/m 2 .

First claim

The invention claimed is:

1. A particulate latex for agglutination assay, comprising:
a first polymerizable monomer having a phenyl group;
a second polymerizable monomer having a phenyl group and a salt of sulfonic acid; and
a third polymerizable monomer represented by Formula (1):
CH 2 ═CR 1 —COOCH 2 CH 2 O(PO 2 )OCH 2 CH 2 —N(CH 3 ) 3   (1)

 where R 1 represents a hydrogen atom or a methyl group,
wherein the density of functional groups of the third polymerizable monomer represented by Formula (1) on the surface of the particulate latex is 0.003 to 0.05 μmol/m 2 .

2. The particulate latex for agglutination assay according to claim 1 , wherein the first polymerizable monomer having the phenyl group is at least one monomer selected from the group consisting of styrene, o-methylstyrene, p-methylstyrene, p-chlorostyrene, and 4-vinylbenzoic acid.

3. The particulate latex for agglutination assay according to claim 1 or 2 , wherein the second polymerizable monomer having the phenyl group and the salt of sulfonic acid is at least one monomer selected from the group consisting of salts of styrenesulfonic acid, salts of divinylbenzene sulfonic acid, salts of o-methylstyrenesulfonic acid, and salts of p-methylstyrenesulfonic acid.

4. The particulate latex for agglutination assay according to claim 1 , wherein the first polymerizable monomer having the phenyl group is styrene, and the second polymerizable monomer having the phenyl group and the salt of sulfonic acid is sodium styrenesulfonate.

5. The particulate latex for agglutination assay according to claim 1 , wherein the particulate latex carries an antigen or an antibody through physical adsorption.

6. A reagent for agglutination assay comprising the particulate latex for agglutination assay according to claim 1 .

Assignees

• Sekisui Medical Co Ltd

Inventors

• Kitahara Shinichiro
• Takahashi Yuki

Classifications

• C08L33/14

  of esters containing halogen, nitrogen, sulfur, or oxygen atoms in addition to the carboxy oxygen · CPC title

• G01N33/545 Primary

  Synthetic resin · CPC title

• C08F290/061 Primary

  Polyesters; Polycarbonates · CPC title

• G01N33/54313

  the carrier being characterised by its particulate form · CPC title

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title