Polymeric carriers for immunohistochemistry and in situ hybridization

Patent metadata

Field	Value
Publication number	US-9103822-B2
Application number	US-201313905918-A
Country	US
Kind code	B2
Filing date	May 30, 2013
Priority date	May 23, 2007
Publication date	Aug 11, 2015
Grant date	Aug 11, 2015

Abstract

Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.

First claim

We claim:

1. A kit comprising a conjugate comprising a specific binding moiety conjugated to a detectable label molecule through a reactive functional group provided by a polymeric carrier comprising plural reactive functional groups selected from hydrazines, hydrazides, hydrazine derivatives, hydrazide derivatives, guanidines, aminoguanidines, hydroxyl amines, or combinations thereof, wherein the molecular weight of the polymeric carrier is between about 1,000 and about 50,000, a…

Assignees

• Ventana Med Syst Inc

Inventors

• Kosmeder Ii Jerry W
• Kernag Casey A
• Johnson Donald
• Bieniarz Christopher

Classifications

• G01N33/5759

  Physics · mapped topic

• G01N33/587

  Physics · mapped topic

• G01N33/54353

  Physics · mapped topic

• G01N33/56983

  Physics · mapped topic

• A61K49/0067

  Human Necessities · mapped topic