Fusion proteins useful for producing pinene

Patent metadata

Field	Value
Publication number	US-9376691-B2
Application number	US-201314091818-A
Country	US
Kind code	B2
Filing date	Nov 27, 2013
Priority date	Nov 28, 2012
Publication date	Jun 28, 2016
Grant date	Jun 28, 2016

Abstract

The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.

First claim

What is claimed is:

1. A fusion protein comprising: (a) a pinene synthase (PS), or enzymatically active fragment thereof, and (b) a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment thereof, wherein the C-terminus of (b) is linked to the N-terminus of (a), and (a) and (b) are linked by a linker which is a peptide of between 3 and 9 amino acid residues; wherein the PS is a Pinus taeda, Picea abies , or Abies grandis PS; and the GPPS is a Picea abies or Abies grandis GPPS.

2. The fusion protein of claim 1 , wherein the linker is a peptide of between 5 and 7 amino acid residues.

3. The fusion protein of claim 2 , wherein the linker is a peptide of 6 amino acid residues.

4. The fusion protein of claim 3 , wherein the peptide of 6 amino acid residues has the amino acid sequence GSGGSG (SEQ ID NO:1).

5. The fusion protein of claim 1 , wherein the PS is a Picea abies or Abies grandis PS.

6. The fusion protein of claim 1 , wherein the GPPS is an Abies grandis GPPS.

7. The fusion protein of claim 1 , wherein the PS and GPPS are from a same species.

8. The fusion protein of claim 7 , wherein the species is Picea abies.

9. The fusion protein of claim 7 , wherein the species is Abies grandis.

Assignees

• Univ California

Inventors

• Peralta-Yahya Pamela P
• Keasling Jay D

Classifications

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12N15/62

  DNA sequences coding for fusion proteins · CPC title

• C12N9/1085

  transferring alkyl or aryl groups other than methyl groups (2.5) · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title