Formation of conjugated protein by electrospinning

Patent metadata

Field	Value
Publication number	US-9371599-B2
Application number	US-201313803873-A
Country	US
Kind code	B2
Filing date	Mar 14, 2013
Priority date	Apr 4, 2012
Publication date	Jun 21, 2016
Grant date	Jun 21, 2016

Abstract

A method of preparing a polysaccharide-protein fiber by preparing an aqueous solution comprising a polysaccharide and a protein, applying a high voltage to the solution, collecting the fiber on a collecting plate.

First claim

What is claimed is:

1. A method of preparing a conjugated polysaccharide-whey protein fiber via electro-spinning comprising steps of
preparing an aqueous solution comprising a polysaccharide and a whey protein, wherein the polysaccharide is present at concentration of 0.1 g/mL to about 5.0 g/mL,
applying a voltage of 15 to 25 kV to the solution,
collecting the fiber on a collecting plate.

2. The method of claim 1 wherein the electro-spinning is needleless.

3. The method of claim 1 wherein the polysaccharide has an aldehyde group or forms an aldehyde group through isomerism.

4. The method of claim 1 wherein the polysaccharide is a dextran.

5. The method of claim 4 wherein the dextran molecular weight is between about 10 kDa and about 500 kDa.

6. The method of claim 1 wherein the whey protein is a whey protein isolate (WPI).

7. The method of claim 1 wherein the aqueous solution comprises polysaccharide and whey protein at a mixing ratio (w/w) from 50:1 to 1:50.

8. The method of claim 1 wherein the aqueous solution comprises polysaccharide and whey protein at a mixing ratio (w/w) from 3:1 to 1:10.

9. The method of claim 1 wherein the aqueous solution comprises dextran and WPI.

10. The method of claim 1 further comprising the step of incubating the fiber at a relative humidity of at least 45%, for up to 24 hours, whereby a conjugated film is formed.

11. The method of claim 10 wherein the relative humidity is between 65% and 75%.

12. The method of claim 10 wherein the temperature is in the range of 10-70° C.

13. The method of claim 1 wherein the fiber diameter is about 100 nm to about 500 nm.

14. The method of claim 13 wherein the fiber diameter is about 150 nm to about 250 nm.

15. A method of preparing a conjugated polysaccharide-protein fiber film by electro-spinning comprising steps of
preparing an aqueous solution comprising 100 kDa dextran and a whey protein isolate, wherein the dextran and the whey protein isolate are present in a mixing ratio (w/w) between 3:1 and 10:1,
applying a voltage of 15 to 25 kV to the solution whereby a fiber is created,
collecting the fiber on a collecting plate, and
incubating the fiber at a relative humidity of at least 45% for between 4 and 24 hours, whereby a conjugated film is formed.

16. The method of claim 15 wherein the relative humidity is between 65% and 75%.

17. The method of claim 15 wherein the incubation is between 4 and 8 hours.

18. The method of claim 15 wherein the temperature is in the range of 10-70° C.

19. The method of claim 15 wherein the electro-spinning is needleless.

Assignees

• Pepsico Inc

Inventors

• Baier Stefan
• Given Peter
• Kanjanapongkul Kobsak
• Weiss Jochen

Classifications

• A23J3/08 Primary

  Dairy proteins · CPC title

• D01F9/00

  Artificial filaments or the like of other substances; Manufacture thereof; Apparatus specially adapted for the manufacture of carbon filaments · CPC title

• D01D5/0076 Primary

  characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid (D01D5/0046 takes precedence) · CPC title

• D01D5/0038 Primary

  the fibre formed by solvent evaporation, i.e. dry electro-spinning · CPC title

• D01F4/00

  Monocomponent artificial filaments or the like of proteins; Manufacture thereof · CPC title