Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use

Patent metadata

Field	Value
Publication number	US-9347046-B2
Application number	US-83838710-A
Country	US
Kind code	B2
Filing date	Jul 16, 2010
Priority date	Jan 22, 2009
Publication date	May 24, 2016
Grant date	May 24, 2016

Abstract

Novel hydroxyphenyl pyruvate dioxygenase (HPPD) polypeptides, variants and fragments thereof, as well as polynucleotides encoding the same, capable of conferring commercial levels of conferring HPPD herbicide resistance or tolerance to plants. Compositions include amino acid sequences, and variants and fragments thereof, for HPPD polypeptides, as well as polynucleotides encoding the same. Methods for the production and use of HPPD herbicide resistant plants that express these novel HPPD polypeptides, methods for selectively controlling weeds in a field at a crop locus, and methods for the assay, characterization, identification and selection of these novel HPPDs are also provided.

First claim

What is claimed is:

1. An polynucleotide encoding a polypeptide having at least 75% identity to SEQ ID NO: 27, wherein said polypeptide is plant-derived and has 4-hydroxyphenyl pyruvate dioxygenase (HPPD) activity and comprises the amino acid sequence (L,I,R)(V,A)(G,A)DVL(S,T) (SEQ ID NO:15), wherein the first L, the I or the R of SEQ ID NO: 15 is replaced with any other amino acid.

2. The isolated polynucleotide of claim 1 , wherein the nucleotide sequence of the isolated polynucleotide is optimized for expression in a plant.

3. An expression cassette comprising the isolated polynucleotide of claim 1 operably linked to a promoter that drives expression in a plant or plant cell.

4. The expression cassette of claim 3 , further comprising an operably linked isolated polynucleotide sequence encoding a polypeptide that confers a desirable trait.

5. The expression cassette of claim 4 , wherein the desirable trait is resistance or tolerance to an herbicide.

6. The expression cassette of claim 5 , wherein said desirable trait is resistance or tolerance to an HPPD inhibitor, glyphosate, or glufosinate.

7. The expression cassette of claim 6 , wherein said polypeptide that confers a desirable trait is a cytochrome P450.

8. The expression cassette of claim 6 , wherein said polypeptide that confers a desirable trait is an EPSPS (5-enol-pyrovyl-shikimate-3-phosphate-synthase).

9. The expression cassette of claim 6 , wherein said polypeptide that confers a desirable trait is a phosphinothricin acetyl transferase (PAT).

10. A vector comprising the expression cassette of claim 3 .

11. A method for conferring resistance or tolerance to an HPPD inhibitor in a plant, the method comprising introducing the expression cassette of claim 3 into the plant.

12. A transformed plant cell comprising the expression cassette of claim 3 .

13. The transformed plant cell of claim 12 , wherein the transformed plant cell is from a plant selected from the group consisting of rice, barley, potato, sweet potato, canola, sunflower, rye, oats, wheat, corn, soybean, sugar beet, tobacco, Miscanthus grass, Switch grass, safflower, trees, cotton, cassava, tomato, sorghum, alfalfa, sugar beet, and sugarcane.

14. The transformed plant cell of claim 12 , wherein the transformed plant cell is a soybean plant cell.

15. A plant, plant part, or seed comprising the plant cell of claim 12 .

16. A method of controlling weeds at a locus comprising crop plants and weeds, wherein the crop plants comprise a plant according to claim 15 , the method comprising applying to the locus a weed-controlling amount of one or more HPPD inhibitors.

17. The method of claim 16 , wherein the one or more HPPD inhibitors are selected from the group consisting of bicyclopyrone benzobicyclon, benzofenap, ketospiradox or its free acid, isoxachlortole, isoxaflutole, mesotrione, pyrasulfotole, pyrazolynate, pyrazoxyfen, sulcotrione, tefuryltrione, tembotrione, topramezone, and agrochemically acceptable salts thereof.

18. The method of claim 16 , wherein the one or more HPPD inhibitors is mesotrione.

19. The isolated polynucleotide of claim 1 , wherein the first L, the I or the R is replaced with E.

20. The isolated polynucleotide of claim 1 , wherein the first L, the I or the R is replaced with C.

21. The isolated polynucleotide of claim 1 , wherein the first L, the I or the R is replaced with A.

22. The isolated polynucleotide of claim 1 , wherein the first L, the I or the R is replaced with D.

Assignees

• Hawkes Timothy Robert
• Langford Michael Phillip
• Viner Russell Colin
• Vernooij Bernardus Theodorus Maria
• Dale Richard
• Singh Shradha
• Kramer Vance
• Syngenta Participations Ag

Inventors

• Hawkes Timothy Robert
• Langford Michael Phillip
• Viner Russell Colin
• Vernooij Bernardus Theodorus Maria
• Dale Richard
• Singh Shradha
• Kramer Vance

Classifications

• C12N9/0069 Primary

  acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13) · CPC title

• A01N41/10

  Sulfones; Sulfoxides · CPC title

• C12N9/0004 Primary

  Oxidoreductases (1.) · CPC title

• A01N43/80

  five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2 · CPC title

• A01N43/40

  six-membered rings · CPC title