Cranberry xyloglucan oligosaccharide composition

Patent metadata

Field	Value
Publication number	US-9314494-B2
Application number	US-201213480903-A
Country	US
Kind code	B2
Filing date	May 25, 2012
Priority date	May 25, 2012
Publication date	Apr 19, 2016
Grant date	Apr 19, 2016

Abstract

A composition prepared from a cranberry hull enzyme-treated composition which reduces or inhibits the adhesion of microorganisms to cells having α-Gal-(1-4)-Gal terminal oligosaccharide receptors for adhesion.

First claim

We claim:

1. An anti-microbial composition, wherein said anti-microbial composition comprises a tablet or capsule having an effective amount of an anti-microbial adhesion inhibitory composition to inhibit the adhesions of bacteria to cells having α-Gal-(1-4)-β-Gal terminal oligosaccharide receptors for adhesion prepared by the method comprising the steps of:
(a) providing cranberry hulls
(b) treating said cranberry hulls with a pectinase in amounts sufficient to depectinize said hulls to form an enzyme treated composition,
(c) removing debris from said enzyme treated composition using a method selected from the group consisting of decantation, centrifugation, and combinations thereof to form a first liquid,
(d) spray drying said first liquid to form composition A1,
(e) fractionating said composition A1 using C18 Flash Chromatography to produce composition A2
(f) freeze drying said composition A2
(g) purifying composition A2 using LH20 gel column chromatography to produce said anti-microbial adhesion inhibitory composition A6,
(h) freeze drying said anti-microbial adhesion inhibitory composition A6 to produce a crystalline powder,
(i) mixing said crystalline powder with a carrier to provide an anti-microbial adhesion inhibitory composition.

2. The composition of claim 1 , wherein said tablet or capsule comprises a medicament, a nutritional composition, or a pharmaceutical composition.

3. An anti-microbial composition, wherein said anti-microbial composition comprises a tablet or capsule having an effective amount of an anti-microbial adhesion inhibitory composition to inhibit the adhesions of bacteria to cells having α-Gal-(1-4)-β-Gal terminal oligosaccharide receptors for adhesion prepared comprising the steps of:
(a) providing cranberry hulls
(b) treating said cranberry hulls with a pectinase in amounts sufficient to depectinize said hulls to form an enzyme treated composition,
(c) removing debris from said enzyme treated composition using a method selected from the group consisting of decantation, centrifugation, and combinations thereof to form a first liquid,
(d) spray drying said first liquid to form composition A1,
(e) fractionating said composition A1 using C18 Flash Chromatography to produce composition A2
(f) freeze drying said composition A2
(g) purifying fraction A2 LH20 using gel column chromatography to produce said anti-microbial adhesion inhibitory composition A6,
(h) freeze drying said anti-microbial adhesion inhibitory composition A6 to produce a crystalline powder,
(i) mixing said crystalline powder with a carrier to provide an anti-microbial adhesion inhibitory composition;
wherein said anti-microbial adhesion inhibitory composition comprises arabino-xyloglucan with an SSG structure:

4. The composition of claim 3 , wherein said tablet or capsule comprises a medicament, a nutritional composition, or a pharmaceutical composition.

5. A method for reducing the adhesion of bacteria to cells having α-Gal-(1-4)-Gal terminal oligosaccharide receptors for adhesion comprising administering to said cells an effective amount of the composition of claim 1 or the composition of claim 3 .

Assignees

• Hotchkiss Arland T
• Nunez Alberto
• Khoo Christina
• Strahan Gary D
• Us Agriculture
• Ocean Spray Cranberries Inc

Inventors

• Hotchkiss Arland T
• Nunez Alberto
• Khoo Christina
• Strahan Gary D

Classifications

• A61P31/04

  Antibacterial agents · CPC title

• A61P13/00

  Drugs for disorders of the urinary system · CPC title

• A61K31/716

  Glucans · CPC title

• A61K36/45 Primary

  Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry · CPC title

• A61K2236/39

  Complex extraction schemes, e.g. fractionation or repeated extraction steps · CPC title