Making c4+ products in bacteria
US-2015376658-A1 · Dec 31, 2015 · US
US9309538B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9309538-B2 |
| Application number | US-200913063362-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 10, 2009 |
| Priority date | Sep 12, 2008 |
| Publication date | Apr 12, 2016 |
| Grant date | Apr 12, 2016 |
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The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of Trichoderma reesei beta-glucosidase, and also relates to the use of said variants having improved effectiveness in methods for cellulose decomposition and biofuel production.
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The invention claimed is: 1. An isolated or purified modified polypeptide comprising an amino acid sequence in which at least one amino acid is modified compared with the amino acid sequence SEQ ID No. 2, said modified amino acid being chosen from positions 225, 238, 240 and 241 of the amino acid sequence SEQ ID No. 2, wherein said modified polypeptide has the amino acid sequence SEQ ID No. 10; or at least 85% identity with said amino acid sequences SEQ ID No. 2 or SEQ ID NO. 10, a…
Cross-Sectional Technologies · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Cross-Sectional Technologies · mapped topic
Chemistry & Metallurgy · mapped topic
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