Staphylococcus aureus proteins and nucleic acids

Patent metadata

Field	Value
Publication number	US-9296796-B2
Application number	US-201414263783-A
Country	US
Kind code	B2
Filing date	Apr 28, 2014
Priority date	Mar 27, 2001
Publication date	Mar 29, 2016
Grant date	Mar 29, 2016

Abstract

The invention provides proteins from Staphylococcus aureus including amino acid sequences and the corresponding nucleotide sequences. The proteins are useful for vaccines, immunogenic compositions, diagnostics, enzymatic studies and also as targets for antibiotics.

First claim

We claim:

1. A composition comprising (1) an immunologically effective amount of an isolated polypeptide comprising (a) an amino acid sequence which has at least 95% sequence identity to SEQ ID NO: 700, or (b) a fragment of SEQ ID NO: 700, wherein the fragment of SEQ ID NO: 700 includes at least ten consecutive amino acids from SEQ ID NO: 700, and (2) a pharmaceutically acceptable excipient, wherein concentration of the isolated polypeptide is at least 1 μg/ml.

2. The composition of claim 1 , wherein the isolated polypeptide comprises SEQ ID NO: 700.

3. The composition of claim 1 , wherein the isolated polypeptide comprises (b) and the fragment includes at least twenty consecutive amino acids from SEQ ID NO: 700.

4. The composition of claim 1 , wherein the isolated polypeptide comprises a hybrid polypeptide represented by the formula NH 2 -A-[-X-L-] n -B-COOH, wherein X is (a) or (b); L is an optional linker amino acid sequence; A is an optional N-terminal amino acid sequence; B is an optional C-terminal amino acid sequence; and n is an integer greater than 1.

5. The composition of claim 1 , further comprising an immunologically effective amount of an adjuvant.

6. The composition of claim 5 , wherein the adjuvant comprises an aluminum salt or an oil-in-water emulsion.

7. The composition of claim 1 , wherein the composition is an immunogenic composition or a diagnostic composition.

8. The composition of claim 1 , further comprising one or more antigens selected from the group consisting of a protein antigen from Helicobacter pylori ; a protein antigen from N. meningitidis serogroup B; an outer-membrane vesicle (OMV) preparation from N. meningitidis ; a saccharide antigen from N. meningitidis serogroup A, C, W135 and/or Y; a saccharide antigen from Streptococcus pneumoniae ; an antigen from hepatitis A virus; an antigen from hepatitis B virus; an antigen from hepatitis C virus; an antigen from Bordetella pertussis ; a diphtheria antigen; a tetanus antigen; a saccharide antigen from Haemophilus influenzae B; an antigen from N. gonorrhoeae ; an antigen from Chlamydia pneumoniae ; an antigen from Streptococcus agalactiae ; an antigen from Streptococcus pyogenes ; an antigen from Chlamydia trachomatis ; an antigen from Porphyromonas gingivalis ; polio antigen(s); rabies antigen(s); measles, mumps and/or rubella antigens; influenza antigen(s); and an antigen from Moraxella catarrhalis.

9. The composition of claim 1 , wherein the isolated polypeptide comprises (b) and the fragment includes at least fifty consecutive amino acids from SEQ ID NO: 700.

10. The composition of claim 6 , wherein the immunologically effective amount of the adjuvant will enhance the immune response to the isolated polypeptide.

11. The composition of claim 1 , further comprising a saccharide antigen conjugated to a carrier protein.

12. The composition of claim 11 , wherein the carrier protein is selected from tetanus toxoid, diphtheria toxoid and CRM 197 .

13. A method of inducing an immune response in a subject comprising administering an immunologically effective amount of the composition of claim 1 to the subject.

Assignees

• Novartis Ag
• Glaxosmithkline Biolog Sa

Inventors

• Masignani Vega
• Mora Marirosa
• Scarselli Maria

Classifications

• A61P31/20

  for DNA viruses · CPC title

• A61K39/085 Primary

  Staphylococcus · CPC title

• C07K14/31 Primary

  from Staphylococcus (G) · CPC title

• A61P31/12

  Antivirals · CPC title

• A61K2039/70

  Multivalent vaccine · CPC title