Self-assembly of macromolecules on multilayered polymer surfaces

Patent metadata

Field	Value
Publication number	US-9274108-B2
Application number	US-27839007-A
Country	US
Kind code	B2
Filing date	Feb 6, 2007
Priority date	Feb 6, 2006
Publication date	Mar 1, 2016
Grant date	Mar 1, 2016

Abstract

The invention is directed toward systems and methods for the formation of two dimensional monolayer structures of ordered biomacromolecules, such as viruses, atop cohesive polyelectrolyte multilayers to create functional thin films. Methods for the formation of such thin films are disclosed that involve an interdiffusion-induced assembly process of the biomacromolecules. The inventive systems provide a general platform for the systematic incorporation and assembly of organic, biological and inorganic materials and will enable many potential technological applications such as, for example, chemical and biological sensors, power devices and catalytic membranes.

First claim

What is claimed is:

1. A composition comprising:
a polyelectrolyte multilayer film, wherein the polyelectrolyte multilayer film comprises a positively-charged polyelectrolyte and a negatively-charged polyelectrolyte; and
a plurality of biomacromolecules, which biomacromolecules are charged and can undergo spontaneous self-assembly, wherein, in the composition, the plurality of biomacromolecules are arranged in an ordered two-dimensional monolayer on a top surface of the polyelectrolyte multilayer film and contact a single polyelectrolyte multilayer film,
wherein the ordered two-dimensional monolayer is a directionally ordered monolayer formed by flowing a solution including the plurality of biomacromolecules in a microfluidic channel.

2. The composition of claim 1 , wherein the biomacromolecules are selected from the group consisting of proteins, polynucleotides, lipids, polysaccharides, and viruses.

3. The composition of claim 1 , wherein the plurality of biomacromolecules comprises a virus.

4. The composition of claim 3 , wherein the virus is a rod-shaped virus.

5. The composition of claim 4 , wherein the virus is a rigid rod-shaped virus.

6. The composition of claim 4 , wherein the virus has a cross-sectional diameter of about 3 nm to about 20 nm and a length of about 60 nm to about 6,000 nm.

7. The composition of claim 3 , wherein the virus comprises at least one recognition site capable of a selective binding to or nucleating of a conjugate moiety.

8. The composition of claim 7 , wherein the virus comprises a first recognition site capable of a first selective binding to or nucleating of a first conjugate moiety, and a second recognition site located differently from the first recognition site capable of a second selective binding to or nucleating of a second conjugate moiety.

9. The composition of claim 8 , wherein the virus further comprises a third recognition site located differently from the first and second recognition sites capable of a third selective binding to or nucleating of a third conjugate moiety.

10. The composition of claim 7 , wherein the virus has been genetically engineered to comprise the at least one recognition site.

11. The composition of claim 10 , wherein the recognition site comprises an expressed protein, peptide or peptide oligomer.

12. The composition of claim 7 , wherein the conjugate moiety is a member of the group consisting of an inorganic material, an organic material, and a biomolecular material.

13. The composition of claim 7 , wherein the conjugate moiety comprises a semiconductor, metallic, magnetic, polymeric, particulate, nanoparticulate, single crystalline, polycrystalline, amorphous, electronically conducting, optically active, conducting polymeric, light-emitting, phosphorescent, fluorescent, glass or ceramic moiety.

14. The composition of claim 1 , wherein the plurality of biomacromolecules are all identical.

15. The composition of claim 1 , wherein the plurality of biomacromolecules comprise different biomacromolecules.

16. The composition of claim 1 , wherein the plurality of biomacromolecules are densely packed in the ordered two-dimensional monolayer.

17. The composition of claim 1 , wherein the plurality of biomacromolecules are sparsely packed in the ordered two-dimensional monolayer.

18. The composition of claim 1 , wherein the two dimensional monolayer of biomacromolecules has a density of about 1 to about 100 biomacromolecules/μm 2 .

19. The composite of claim 1 , wherein the positively-charged polyelectrolyte is a weak positively-charged polyelectrolyte.

20. The composite of claim 1 , wherein the negatively-charged polyelectrolyte is a weak negatively-charged polyelectrolyte.

21. The composition of claim 1 , wherein the positively-charged polyelectrolyte is a weak positively-charged polyelectrolyte and the negatively-charged polyelectrolyte is a weak negatively-charged polyelectrolyte, and they form a pair of weak oppositely charged polyelectrolytes.

22. The composition of claim 21 , wherein the polyelectrolytes have a surface charge density that is higher than the surface charge density of the biomacromolecules.

23. The composition of claim 1 , wherein the positively-charged polyelectrolyte and/or the negatively-charged polyelectrolyte is/are biocompatible.

24. The composition of claim 1 , wherein the positively-charged polyelectrolyte and/or the negatively-charged polyelectrolyte is/are degradable.

25. A method of preparing the composition of claim 1 , the method comprising the steps of:
providing the plurality of biomacromolecules;
providing the polyelectrolyte multilayer film produced by layer-by-layer assembly using the positively-charged polyelectrolyte and the negatively-charged polyelectrolyte, wherein the top surface of the film is positively charged; and
contacting the top surface of the film with the plurality of biomacromolecules by flowing the solution including the plurality of biomacromolecules in the microfluidic channel.

26. The method of claim 25 , wherein the solution including the plurality of biomacromolecules is an aqueous solution.

27. The method of claim 26 , wherein the aqueous solution including the plurality of biomacromolecules has a pH that is selected such that strong repulsion takes place between the biomacromolecules.

28. The method of claim 26 , wherein the aqueous solution including the plurality of biomacromolecules has a pH that is selected such that weak repulsion takes place between the biomacromolecules.

29. The composition of claim 1 , wherein a density of the ordered two-dimensional monolayer increases with an increase in a pH of a polyelectrolyte solution.

Assignees

• Yoo Pil J
• Nam Ki Tae
• Qi Jifa
• Lee Soo-Kwan
• Park Juhyun
• Belcher Angela M
• Hammond-Cunningham Paula T
• Massachusetts Inst Technology

Inventors

• Yoo Pil J
• Nam Ki Tae
• Qi Jifa
• Lee Soo-Kwan
• Park Juhyun
• Belcher Angela M
• Hammond-Cunningham Paula T

Classifications

• Y10T428/31971

  Of carbohydrate · CPC title

• Y10T428/273

  of coating · CPC title

• Y10T428/31855

  Of addition polymer from unsaturated monomers · CPC title

• Y10T156/10

  Methods of surface bonding and/or assembly therefor · CPC title

• G01N33/545 Primary

  Synthetic resin · CPC title