Assay method using encoded particle-based platform

Patent metadata

Field	Value
Publication number	US-9267950-B2
Application number	US-201414153159-A
Country	US
Kind code	B2
Filing date	Jan 13, 2014
Priority date	May 17, 2011
Publication date	Feb 23, 2016
Grant date	Feb 23, 2016

Abstract

Provided is an assay method using an encoded particle-based platform. In the assay method, first, a plurality of encoded particles having codes distinguishable from one another according to kinds of included target materials are prepared. The plurality of encoded particles are provided onto a plate including a plurality of wells by pipetting, and disposed in the plurality of wells by a self-assembly method. An analyte is provided into the plurality of wells. The codes of the plurality of encoded particles disposed in the plurality of wells are decoded. The target materials of the plurality of encoded particles are released to cause a reaction between the target materials and the analyte.

First claim

What is claimed is:

1. An assay method for determining target materials reacting with an analyte, comprising the steps of:
preparing a plurality of encoded particles including the target materials, the plurality of encoded particles having one or more codes distinguishable from one another corresponding to kinds of the included target materials, wherein each target material is a chemical material or a biological material;
preparing a plate including wells, wherein at least one support is formed in each well, the support forming a space between a bottom of the well and a top of the support;
providing the plurality of encoded particles onto the plate by pipetting, and disposing the plurality of encoded particles in the wells, whereby each encoded particle is place on the top of the support;
providing the analyte into the space formed in each well, wherein the analyte is a biological material or a chemical material;
releasing the target materials of the plurality of encoded particles to cause a reaction between the target materials and the analyte, wherein the target materials are released by a physical cue, the physical cue comprising at least one of a pressure applied from outside the plate and heating, wherein the target materials are released from the plurality of encoded particles to contact the analyte;
decoding the codes of the plurality of encoded particles; and
determining the target materials reacting with the analyte.

2. The method of claim 1 , wherein the chemical material is a drug, and
the biological material is selected from the group consisting of cells, molecules, protein, bacteria, deoxyribonucleic acid (DNA), and ribonucleic acid (RNA).

3. The method of claim 1 , wherein a fluid as a carrier is used in the step of providing the plurality of encoded particles onto the plate.

4. The method of claim 3 , wherein the fluid is selected from the group consisting of an alcohol, water, phosphate buffered saline (PBS), and an oil.

5. The method of claim 1 , wherein the step of providing the plurality of encoded particles onto the plate comprises putting the plurality of encoded particles in a fluid and the fluid carries the encoded particle as a carrier, and
a concentration of the plurality of encoded particles in the fluid is determined by a number of the plurality of encoded particles included in the fluid.

6. The method of claim 5 , wherein the fluid is selected from the group consisting of an alcohol, water, phosphate buffered saline (PBS), and an oil.

7. The method of claim 1 , wherein the pressure applied from outside the plate is provided by a grinder.

8. The method of claim 1 , wherein the heating is provided by a laser.

Assignees

• Snu R&Db Foundation

Inventors

• Kwon Sung Hoon
• Chung Su Eun
• Lee Sung Hoon
• Park Wook
• Song Young Hoon

Classifications

• G01N33/54313 Primary

  the carrier being characterised by its particulate form · CPC title

• G01N33/585 Primary

  with a particulate label, e.g. coloured latex · CPC title

• Y10T436/143333

  Saccharide [e.g., DNA, etc.] · CPC title

• G01N35/10

  Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} · CPC title

• G01N33/48 Primary

  Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) · CPC title