Antibody and recombinant derivative for the detection of trinitrotoluene

Patent metadata

Field	Value
Publication number	US-9267930-B2
Application number	US-201213653474-A
Country	US
Kind code	B2
Filing date	Oct 17, 2012
Priority date	Oct 17, 2012
Publication date	Feb 23, 2016
Grant date	Feb 23, 2016

Abstract

Described herein are new recognition elements (antibodies or functional fragments thereof) that effectively bind to trinitrotoluene (TNT). Also disclosed is a single chain fragment recognition element.

First claim

What is claimed is:

1. An isolated antibody or functional fragment thereof that binds to trinitrotoluene, comprising a heavy chain having a sequence of SEQ ID NO:1 and a light chain having a sequence of SEQ ID NO:2.

2. The isolated antibody or functional fragment thereof according to claim 1 , wherein the antibody or functional fragment thereof is a single chain variable fragment.

3. The isolated antibody or functional fragment thereof according to claim 1 , wherein the isolated antibody or functional fragment thereof is a single chain antibody, wherein the combined heavy and light chain have a sequence of SEQ ID NO:3.

4. An isolated nucleic acid molecule comprising a nucleotide encoding the isolated antibody or functional fragment thereof according to claim 1 .

5. A host cell in vitro transformed with the isolated nucleic acid molecule according to claim 4 .

6. A method of producing an antibody or functional fragment thereof, the method comprising culturing host cells according to claim 5 under conditions for production of the antibody or functional fragment thereof.

7. The method according to claim 6 , further comprising isolating and/or purifying the antibody or functional fragment thereof.

8. A method of detecting trinitrotoluene (TNT), the method comprising reacting a sample known or suspected of containing TNT with the isolated antibody or functional fragment thereof according to claim 1 .

9. An apparatus configured to detect trinitrotoluene (TNT), the apparatus comprising the isolated antibody or functional fragment thereof according to claim 1 .

Assignees

• Liu Jinny Lin
• Zabetakis Daniel
• Goldman Ellen R
• Anderson George P
• Us Navy

Inventors

• Liu Jinny Lin
• Zabetakis Daniel
• Goldman Ellen R
• Anderson George P

Classifications

• C07K2317/56

  variable (Fv) region, i.e. VH and/or VL · CPC title

• C07K2317/94

  Stability, e.g. half-life, pH, temperature or enzyme-resistance · CPC title

• C07K2317/92

  Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value · CPC title

• G01N33/5308

  for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title

• C07K2317/569

  Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody® · CPC title