Nanoliter array loading

Patent metadata

Field	Value
Publication number	US-9266108-B2
Application number	US-201314042458-A
Country	US
Kind code	B2
Filing date	Sep 30, 2013
Priority date	Mar 12, 2004
Publication date	Feb 23, 2016
Grant date	Feb 23, 2016

Abstract

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

First claim

What is claimed is:

1. A system for holding a plurality of biomolecular samples, comprising:
a receptacle comprising a plurality of wells disposed within a substrate;
a plurality of sample chips disposed inside respective wells of the receptacle, each chip comprising an array of sample wells and
a loader interface structure comprising an upper portion and a lower portion, the lower portion configured to hold the sample chips, wherein the upper portion is disposed above the plurality of wells of the receptacle while the lower portion is disposed inside at least some of the plurality of wells of the receptacle.

2. The system of claim 1 , wherein the receptacle comprises a microtiter plate.

3. The system of claim 1 , wherein at least one of the chips comprises an array of through-hole sample wells.

4. The system of claim 1 , wherein at least some of the sample wells of at least one of the chips comprise a through-hole sample well.

5. The system of claim 1 , wherein at least some of the sample wells have a volume of 0.2 nanoliter to 100 nanoliters.

6. The system of claim 1 , wherein at least some of the sample chips comprise 1000 or more sample wells.

7. The system of claim 1 , further comprising a fluid introduction mechanism configured to introduce a biomolecular sample fluid from at least some of the wells of the receptacle and into at least some of the sample wells.

8. A kit for holding a plurality of biomolecular samples, comprising:
a receptacle comprising a plurality of wells disposed within a substrate;
a plurality of sample chips, each chip comprising an array of sample wells;
the plurality of sample chips configured to fit inside respective wells of the receptacle and
a loader interface structure comprising an upper portion and a lower portion, the lower portion configured to hold the sample chips, wherein during use the upper portion is disposed above the plurality of wells of the receptacle while the lower portion is disposed inside at least some of the plurality of wells of the receptacle.

9. The kit of claim 8 , wherein the receptacle comprises a microtiter plate.

10. The kit of claim 8 , wherein at least one of the chips comprises an array of through-hole sample wells.

11. The kit of claim 8 , wherein at least some of the sample wells of at least one of the chips comprise a through-hole sample well.

12. The kit of claim 8 , wherein at least some of the sample wells have a volume of 0.2 nanoliter to 100 nanoliters.

13. The kit of claim 8 , wherein at least some of the sample chips comprise 1000 or more sample wells.

14. The kit of claim 8 , further comprising a fluid introduction mechanism configured to introduce a biomolecular sample fluid from at least some of the wells of the receptacle and into at least some of the sample wells.

15. A method for holding a plurality of biomolecular samples, comprising:
providing a receptacle comprising a plurality of wells disposed within a substrate;
providing a loader interface structure comprising an upper portion and a lower portion, the lower portion holding a plurality of sample chips; and
placing the plurality of sample chips inside respective wells of the receptacle, each chip comprising an array of through-hole sample wells
wherein placing the plurality of chips comprises locating the upper portion above the plurality of wells of the receptacle and locating the lower portion inside at least some of the plurality of wells of the receptacle.

16. The method of claim 15 , wherein the receptacle comprises a microtiter plate.

17. The method of claim 15 , further comprising introducing a biomolecular sample from at least some of the wells of the receptacle into at least some of the sample wells.

Assignees

• Life Technologies Corp

Inventors

• Kanigan Tanya S
• Smith Steve
• Linton John
• Hess Robert
• Yoder Karl
• Brenan Colin John Herbert

Classifications

• B01L3/0244 Primary

  using pins · CPC title

• Y10T137/0318

  Processes · CPC title

• B01L2200/0642

  Filling fluids into wells by specific techniques · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• B01L3/563

  Joints or fittings; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors · CPC title