Method of deriving mature hepatocytes from human embryonic stem cells
US-9512428-B2 · Dec 6, 2016 · US
Method for producing epithelial stem cells
US9249394B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9249394-B2 |
| Application number | US-201214007133-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 23, 2012 |
| Priority date | Mar 25, 2011 |
| Publication date | Feb 2, 2016 |
| Grant date | Feb 2, 2016 |
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Abstract
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An object of this invention is to provide a method, etc., for efficiently proliferating a pluripotent epithelial somatic stem cell. A method for producing an epithelial somatic stem cell comprising Steps (A) and (B) below: (A) expressing a gene of a protein having an activity for causing a cell in G0 phase or G1 phase to enter S phase, in a cell population including an epithelial somatic stem cell; and (B) culturing the cell obtained in Step (A) in the presence of an extracellular growth factor.
First claim
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The invention claimed is: 1. A method for producing an epithelial somatic stem cell, comprising: (A) expressing a gene of a protein having an activity for causing a cell in G0 phase or G1 phase to enter S phase, in a cell population comprising an epithelial somatic stem cell; and (B) culturing the cell obtained in the Step (A) in the presence of an extracellular growth factor, wherein the protein is a cyclin-dependent kinase, and wherein the cell population is a hepatic cell population. 2. The method according to claim 1 , wherein the expression is transient expression. 3. The method according to claim 1 , wherein Step (A) and Step (B) are repeated at least twice. 4. The method according to claim 1 , wherein the protein is at least one protein selected from the group consisting of cyclin-dependent kinase 4 and cyclin-dependent kinase 6. 5. The method according to claim 1 , wherein the cell population is a primary-cultured somatic cell population. 6. The method according to claim 1 , wherein the extracellular growth factor is a hepatocyte growth factor (HGF). 7. The method according to claim 1 , wherein the protein is at least one protein selected from the group consisting of cyclin-dependent kinase 1, cyclin-dependent kinase 2, cyclin-dependent kinase 3, cyclin-dependent kinase 4, cyclin-dependent kinase 6, and cyclin-dependent kinase 7.
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Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title
Genetically modified cells · CPC title
transferring phosphorus containing groups, e.g. kinases (2.7) · CPC title
Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor · CPC title
containing a FLAG-tag · CPC title
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- What does patent US9249394B2 cover?
- An object of this invention is to provide a method, etc., for efficiently proliferating a pluripotent epithelial somatic stem cell. A method for producing an epithelial somatic stem cell comprising Steps (A) and (B) below: (A) expressing a gene of a protein having an activity for causing a cell in G0 phase or G1 phase to enter S phase, in a cell population including an epithelial somat…
- Who is the assignee on this patent?
- Hijikata Makoto, Aly Hassan Hussein, Yamaguchi Tatsuya, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0672. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 02 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).