Method of deriving mature hepatocytes from human embryonic stem cells
US-9512428-B2 · Dec 6, 2016 · US
Adipose derived adult stem cells in hepatic regeneration
US2016237408A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016237408-A1 |
| Application number | US-201615012061-A |
| Country | US |
| Kind code | A1 |
| Filing date | Feb 1, 2016 |
| Priority date | Apr 25, 2006 |
| Publication date | Aug 18, 2016 |
| Grant date | — |
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Abstract
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The present invention provides a method to derive hepatic stem cells from stem cells derived from non-liver tissue. In one embodiment of the invention, hepatic stem cells are derived from adipose stem cells. The invention also provides a method of enhancing hepatic cytokine production (e.g., HGF) from ASCs, which may be useful in the regeneration of liver tissue when transplanted in vivo. Tissue culture conditions, including media conditions, are provided.
First claim
Opening claim text (preview).
What is claimed is: 1 . A method of deriving hepatic stem cells from non-liver stem cells comprising: (a) providing stem cells derived from non-liver tissue; and (b) culturing the stem cells on an extracellular matrix comprising at least one fibrilar matrix protein and in serum-free culture medium comprising hepatic growth factor (HGF), in which the stem cells derived from non-liver tissue differentiate into hepatic stem cells. 2 . The method according to claim 1 in which the non-liver tissue is adipose tissue. 3 . The method according to claim 1 in which the non-liver tissue is tissue from an adult mammal. 4 . The method according to claim 1 in which the at least one fibrilar matrix protein is selected from the group consisting of collagen I and collagen III. 5 . The method according to claim 4 in which the at least one fibrilar matrix protein is collagen I. 6 . The method according to claim 1 in which the culture medium further comprises Oncostatin M, DMSO or both. 7 . The method according to claim 1 in which the extracellular matrix further comprises fibronectin, matrigel, vitrogen or combinations thereof. 8 . The method according to claim 1 in which greater than about 70% of the stem cells derived from non-liver tissue differentiate into hepatic stem cells. 9 . The method of claim 1 in which the stem cells derived from non-liver tissue were further derived from passage in culture. 10 . A method of inducing HGF expression from non-liver stem cells comprising: (a) providing stem cells derived from non-liver tissue; and (b) culturing the stem cells on an extracellular matrix comprising at least one fibrilar matrix protein and in serum-free culture medium comprising EGF, bFGF, or both, in which the stem cells derived from non-liver tissue produce HGF. 11 . The method of claim 10 in which the culture medium comprises EGF and bFGF. 12 . The method of claim 10 in which the culture medium further comprises sodium ascorbic diphosphate. 13 . The method of claim 11 in which the culture medium further comprises sodium ascorbic diphosphate.
Assignees
Inventors
Classifications
Collagen; Gelatin · CPC title
Epidermal growth factor [EGF] · CPC title
Organic phosphate, e.g. beta glycerophosphate · CPC title
- C12N5/0672Primary
Stem cells; Progenitor cells; Precursor cells; Oval cells · CPC title
Serum-free medium, which may still contain naturally-sourced components · CPC title
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- What does patent US2016237408A1 cover?
- The present invention provides a method to derive hepatic stem cells from stem cells derived from non-liver tissue. In one embodiment of the invention, hepatic stem cells are derived from adipose stem cells. The invention also provides a method of enhancing hepatic cytokine production (e.g., HGF) from ASCs, which may be useful in the regeneration of liver tissue when transplanted in vivo. Tissu…
- Who is the assignee on this patent?
- Univ Louisiana State, Artecel Inc, Univ Louisiana State
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0672. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Aug 18 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).