Vitamin d measurement method and measurement kit
US-2015355202-A1 · Dec 10, 2015 · US
US9201079B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9201079-B2 |
| Application number | US-201013503963-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 19, 2010 |
| Priority date | Oct 26, 2009 |
| Publication date | Dec 1, 2015 |
| Grant date | Dec 1, 2015 |
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Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise associating an amine with a dihydroxyvitamin D metabolite in a sample, ionizing the adduct, and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample.
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What is claimed is: 1. A method for quantifying the amount of a dihydroxyvitamin D metabolite in a sample, comprising the steps of: (a) combining a first sample comprising an amount of dihydroxyvitamin D metabolite with an amine selected from the group consisting of glycine, alanine, serine, arginine, thyroxine, triiodothyronine, diiodothyronine and iodothyronine, thereby forming a second sample comprising non-covalent adducts comprising the dihydroxyvitamin D metabolite and the…
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