Biomaterial test apparatus and method of controlling the same
US-2016325281-A1 · Nov 10, 2016 · US
Channels with cross-sectional thermal gradients
US9222115B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9222115-B2 |
| Application number | US-201213724503-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 21, 2012 |
| Priority date | Dec 30, 2011 |
| Publication date | Dec 29, 2015 |
| Grant date | Dec 29, 2015 |
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Abstract
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Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone. Such devices find use in, for example, nucleic acid amplification procedures, including digital polymerase chain reaction (dPCR) to temperature cycle droplets for amplification of nucleic acid templates within the droplets.
First claim
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We claim: 1. A device comprising: a) a microchannel comprising a surface configured for transmission of a carrier medium comprising droplets; b) a first thermal layer positioned along said microchannel configured to heat a portion of said microchannel surface to a nucleic acid denaturation temperature; and c) a second thermal layer positioned along said microchannel configured to heat or cool a portion of said microchannel surface to a nucleic acid annealing temperature; whe…
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- What does patent US9222115B2 cover?
- Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid anne…
- Who is the assignee on this patent?
- Abbott Molecular Inc, Abbott Molecular Inc
- What technology area does this patent fall under?
- Primary CPC classification B01L7/54. Mapped technology areas include Operations & Transport.
- When was this patent published?
- Publication date Tue Dec 29 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).