Manipulation of fluids and reactions in microfluidic systems
US-9017623-B2 · Apr 28, 2015 · US
US9440232B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9440232-B2 |
| Application number | US-201414577652-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 19, 2014 |
| Priority date | Feb 6, 2007 |
| Publication date | Sep 13, 2016 |
| Grant date | Sep 13, 2016 |
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Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.
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What is claimed is: 1. A method, comprising: providing a microfluidic network comprising five or more sections in a serial arrangement, each section comprising a first microchannel, a second microchannel, a microwell, and a junction, wherein the junction is fluidicly coupled to the first and second microchannels and the microwell, and the microwell comprises a constricted fluidic path that exits the microwell; flowing an oil in the microfluidic network; flowing a plurality of…
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