Processing biomass
US-2016369315-A1 · Dec 22, 2016 · US
D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of D-psicose using same
US9217166B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9217166-B2 |
| Application number | US-201214240647-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 21, 2012 |
| Priority date | Aug 24, 2011 |
| Publication date | Dec 22, 2015 |
| Grant date | Dec 22, 2015 |
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Abstract
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The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.
First claim
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The invention claimed is: 1. A D-psicose 3-epimerase variant with improved thermostability, wherein the variant is a variant of a wild type D-psicose 3-epimerase of SEQ ID NO:1 derived from Agrobacterium tumefaciens and has an amino acid sequence in which isoleucine (Ile) at position 33 of the amino acid sequence of SEQ ID NO:1 is substituted with an amino acid selected from the group consisting of leucine (Leu), cysteine (Cys) and valine (Val), or an amino acid sequence in which…
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- What does patent US9217166B2 cover?
- The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant …
- Who is the assignee on this patent?
- Kim Yang Hee, Kim Jin Ha, Lee Young Mi, and 8 more
- What technology area does this patent fall under?
- Primary CPC classification C12P19/24. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 22 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).