Lactobacillus paracasei strain
US-12152232-B2 · Nov 26, 2024 · US
US9504271B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9504271-B2 |
| Application number | US-201414311185-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 20, 2014 |
| Priority date | Dec 23, 2011 |
| Publication date | Nov 29, 2016 |
| Grant date | Nov 29, 2016 |
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A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.
Opening claim text (preview).
What is claimed is: 1. A method for sterilizing microbial cells, the method comprising: cultivating microbial cells comprising genus Corynebacterium in a culture such that O.D. 600 becomes at least 0.6; and subsequently, adding to the culture an amount of polyoxyethylene fatty acid amide to effectively sterilize the microbial cells to a survival rate of 10 −5 or less, wherein the amount is in a concentration of 0.5 to 8% w/w with reference to the culture.…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
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