Detecting objects

Patent metadata

Field	Value
Publication number	US-9212985-B2
Application number	US-52387808-A
Country	US
Kind code	B2
Filing date	Jan 22, 2008
Priority date	Jan 22, 2007
Publication date	Dec 15, 2015
Grant date	Dec 15, 2015

Abstract

The invention provides apparatus and methods for detecting objects in samples. The sample is held in the transmission path of light from a light source to a detector, whereby light from the light source interacts with objects in the sample. The patterns of light incident on the detector subsequent to its interaction with the objects are directly used to determine the presence of objects in the sample.

First claim

The invention claimed is:

1. Apparatus for detecting objects in a sample, the apparatus comprising:
an incoherent point light source;
a detector having an active light detecting surface positioned and configured to detect diffraction or interference light patterns incident on the said surface, produced by light from the point light source which extends along a path which expands laterally outwardly from the point light source to said surface, along which path it interacts with objects in a sample;
a sample holding location spaced from the said surface at which the sample is held in a sample holder or holders in the transmission path of light from the point light source to the said surface, the distance from the sample holding location to the said surface being less than the distance from the point light source to the sample holding location and allowing the light from the point light source, upon interacting with the objects in the sample to form said diffraction or interference light patterns at the said surface; and
a processor configured to receive input from the detector indicative of the said diffraction or interference light patterns incident on the detector at the said surface, and processing said input to determine the presence of an object within the sample directly from the said incident diffraction or interference light patterns.

2. Apparatus according to claim 1 , comprising a plurality of additional incoherent point light sources spaced from one another, light from each of the point light sources being incident on the detector subsequent to passing through the sample holding location, and wherein the apparatus further comprises a light source controller for controlling the point light sources such that not all of the point light sources emit light simultaneously.

3. Apparatus according to claim 2 , wherein the light source controller controls the point light sources to emit light sequentially.

4. Apparatus according to claim 1 , wherein at least one incoherent point light source is apertured to a diameter of no more than 100 μm.

5. Apparatus according to claim 1 , wherein at least one incoherent point light source is a light emitting diode.

6. Apparatus according to claim 1 , wherein at least one incoherent point light source is an optical fibre.

7. Apparatus according to claim 1 , wherein the said surface is a pixelated array.

8. Apparatus according to claim 7 , wherein the detector is a CCD or CMOS detector.

9. Apparatus according to claim 1 , wherein the processor is arranged to determine the spatial location within the sample holding location of a detected object from the said input of the incident light patterns.

10. Apparatus according to claim 9 , wherein the spatial location is determined in two dimensions in a plane parallel to the plane of said surface.

11. Apparatus according to claim 9 , wherein the spatial location is determined in three dimensions.

12. Apparatus according to claim 3 , wherein the processor is arranged to determine the spatial location in three dimensions within the sample holding area of a detected object from the said input of the incident light patterns.

13. Apparatus according to claim 1 , wherein the detector is arranged to capture a series of two or more sequential light patterns over a period of time and the processor is arranged to analyze the series of light patterns to determine whether the location of a detected object at the sample holding location has changed.

14. Apparatus according to claim 13 , wherein the processor is arranged to record a sequence of locations of an object within the sample holding location to determine the trajectory of the cell or bacterium.

15. Apparatus according to claim 1 , wherein the detector is arranged to capture a series of two or more sequential light patterns over a period of time and the processor is arranged to analyze the series of light patterns to detect a change in the light pattern associated with a specific detected object, indicating a change in relation to a detected object other than a change in its location.

16. Apparatus according to claim 15 , wherein the object is a cell and the change is cell attachment or cell mitosis or cell death.

17. Apparatus according to claim 1 , wherein the processor is arranged to analyze the detected light patterns to count the number of detectable objects in the sample.

18. Apparatus according to claim 1 , wherein the detected light is in the form of diffraction patterns.

19. Apparatus according to claim 1 , wherein the detected light is in the form of interference patterns.

20. A microfluidic platform comprising apparatus for detecting objects according to claim 1 .

21. Apparatus for detecting objects in a plurality of samples, the apparatus comprising a plurality of sample holders and a detecting apparatus according to claim 1 .

22. Apparatus according to claim 21 , wherein the plurality of sample holders are provided by a multi-well plate.

23. Apparatus according to claim 21 , the detecting apparatus being movable relative to said plurality of sample holders to sequentially bring said sample holders into the sample holding location of the detecting apparatus.

24. Apparatus according to claim 21 , comprising a plurality of said detecting apparatus, whereby a plurality of said sample holders can be located in respective sample holding location of the detecting apparatus simultaneously.

25. Apparatus according to claim 1 , wherein said objects are cells or bacteria.

26. An apparatus according to claim 1 , wherein the apparatus is a hand held device.

27. An apparatus according to claim 26 , where the hand held device is a blood cell counter.

28. An apparatus according to claim 1 , including a plurality of point light sources, each at a different wavelengths.

29. An apparatus according to claim 28 , wherein the different light sources can be red, green or blue for color imaging.

30. An apparatus according to claim 28 , where the different light sources can include ultra violet for fluorescence excitation.

31. An apparatus according to claim 1 , wherein the processor is configured to derive features of the cells using signal processing methods.

32. An apparatus according to claim 1 , wherein the detected light source is fluorescence.

Assignees

• Vojnovic Borivoj
• Barber Paul Richard
• Pope Iestyn
• Smith Paul James
• Errington Rachel Jane
• Isis Innovation

Inventors

• Vojnovic Borivoj
• Barber Paul Richard
• Pope Iestyn
• Smith Paul James
• Errington Rachel Jane

Classifications

• G01N15/1456 Primary

  without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals · CPC title

• G03H1/0443

  Digital holography, i.e. recording holograms with digital recording means (holobject computation G03H1/0866) · CPC title

• G03H2001/0825

  Numerical processing in hologram space, e.g. combination of the CGH [computer generated hologram] with a numerical optical element · CPC title

• G01N21/4788

  Diffraction (for sizing particles G01N15/0205) · CPC title

• G03H2222/34

  Multiple light sources · CPC title