Methods and Systems for Image Data Processing

Patent metadata

Field	Value
Publication number	US-2016267315-A1
Application number	US-201615069037-A
Country	US
Kind code	A1
Filing date	Mar 14, 2016
Priority date	Sep 21, 2005
Publication date	Sep 15, 2016
Grant date	—

Abstract

Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

First claim

1 - 7 . (canceled)

8 . A system, comprising:
an imaging subsystem configured to image, at different wavelength bands, particles disposed within the imaging subsystem; and a data processing subsystem configured to:
store data acquired for multiple images of the particles, wherein the multiple images correspond to different wavelength bands, and wherein particular images of the multiple images include spots corresponding to the particles;
create a first composite image of the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images; and
modify coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images.

9 . The system of claim 8 , wherein the modifying includes performing an orthogonal offset of the coordinates.

10 . The system of claim 8 , wherein the modifying includes performing a rotation of the at least one of the multiple images.

11 . The system of claim 8 , wherein the modifying includes:
determining an offset such that an amount of color variance in the second composite image is less than an amount of color variance in the first composite image.

12 . The system of claim 11 , wherein the data processing subsystem is further configured to iteratively create a series of composite images until a particular one of the series of composite images has a color variance below a threshold color variance.

13 . The system of claim 8 , wherein the modifying includes:
based on a plurality of predetermined offsets, determining an aggregate error or a mean squared difference among pixels corresponding to the particles within the first composite image; and selecting a predetermined offset by minimizing the aggregate error or mean squared difference.

14 . The system of claim 8 , wherein the data processing subsystem is further configured to iteratively create a plurality of composite images until a threshold amount of misalignment is reached.

15 . A method, comprising:
a computing system receiving data corresponding to multiple images of a plurality of particles, wherein the multiple images correspond to different wavelength bands, and wherein particular images of the multiple images include spots corresponding to individual ones of the plurality of particles; the computing system creating a first composite image based on the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images; the computing system modifying coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images; and the computing system outputting the second composite image.

16 . The method of claim 15 , wherein the particles include fluorescent material, and wherein the images are based on fluorescence from the fluorescent material.

17 . The method of claim 16 , wherein the particles include at least two distinct fluorescent materials.

18 . The method of claim 15 , wherein the particles include at least one of: a microparticle, a nanoparticle, a quantum dot, or a cell.

19 . The method of claim 15 , wherein the particles include at least one of: a polystyrene bead or a latex bead.

20 . The method of claim 15 , wherein at least some of the particles emit light in a classification wavelength band.

21 . The method of claim 20 , wherein the at least some of the particles also emit light in a reporter wavelength band.

22 . An article of manufacture including a computer-readable medium having instructions coded thereon that, in response to execution by a computing system, cause the computing system to carry out operations comprising:
acquiring data corresponding to multiple images of a plurality of particles, wherein the multiple images correspond to different wavelength bands, and wherein particular images of the multiple images include spots corresponding to individual ones of the plurality of particles; creating a first composite image based on the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images; and modifying coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images.

23 . The article of claim 22 , wherein the operations further comprise:
determining whether a selected particle that has been imaged at first wavelength band has also been imaged at a second wavelength band; in response to the selected particle being imaged at the second wavelength band, accepting the selected particle for further evaluation; and in response to the selected particle not being imaged at the second wavelength band, rejecting the selected particle for further evaluation.

24 . The article of claim 22 , wherein the modifying includes modifying coordinates of all of the multiple images except for one reference image.

25 . The article of claim 24 , wherein the reference image is based on fluorescence from a classification dye.

26 . The article of claim 22 , wherein the modifying is based on a color variance among the first composite spots.

27 . The article of claim 26 , wherein the modifying is repeated until the color variance is less than a threshold color variance.

Assignees

• Luminex Corp

Inventors

• Roth Wayne D

Classifications

• G06F18/00

  Pattern recognition · CPC title

• G06K9/0014 Primary

  Physics · mapped topic

• G06T2207/10064

  Fluorescence image · CPC title

• G06T2207/20021

  Dividing image into blocks, subimages or windows · CPC title

• G06T2207/30024

  Cell structures in vitro; Tissue sections in vitro · CPC title