Comparative analysis of protein conformations by using 2D NOESY NMR spectra

US9182467B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9182467-B2
Application numberUS-59586708-A
CountryUS
Kind codeB2
Filing dateApr 15, 2008
Priority dateApr 16, 2007
Publication dateNov 10, 2015
Grant dateNov 10, 2015

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Abstract

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The present disclosure provides a method for determining the relative conformations of a protein provided in different protein preparations, comprising steps of: (i) obtaining a first 2D NOESY NMR spectrum of a first protein preparation; (ii) obtaining a second 2D NOESY NMR spectrum of a second protein preparation; and (iii); determining whether a protein has a different conformation in the first and second protein preparations by comparing one or more cross-peaks in the first 2D NOESY NMR spectrum with one or more corresponding cross-peaks in the second 2D NOESY NMR spectrum.

First claim

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We claim: 1. A method of measuring differences in relative conformations of a recombinant glycoprotein provided in different preparations, comprising steps of: generating a first 2D NOESY NMR spectrum of a first preparation of a recombinant glycoprotein using an NMR spectrometer, wherein the recombinant glycoprotein has been recombinantly expressed in a mammalian cell; generating a second 2D NOESY NMR spectrum, comparable to the first spectrum, of a second preparation of the sa…

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What does patent US9182467B2 cover?
The present disclosure provides a method for determining the relative conformations of a protein provided in different protein preparations, comprising steps of: (i) obtaining a first 2D NOESY NMR spectrum of a first protein preparation; (ii) obtaining a second 2D NOESY NMR spectrum of a second protein preparation; and (iii); determining whether a protein has a different conformation in the fir…
Who is the assignee on this patent?
Parsons Ian Christopher, Lansing Jonathan, Bosques Carlos J, and 2 more
What technology area does this patent fall under?
Primary CPC classification G01R33/4633. Mapped technology areas include Physics.
When was this patent published?
Publication date Tue Nov 10 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).