Stable isotope-labeled aliphatic amino acid and NMR structural analysis of protein using same

US9739732B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9739732-B2
Application numberUS-201314421814-A
CountryUS
Kind codeB2
Filing dateFeb 18, 2013
Priority dateAug 16, 2012
Publication dateAug 22, 2017
Grant dateAug 22, 2017

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Abstract

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Provided is a stable isotope-labeled aliphatic amino acid enabling the assignment of the signal of an amino acid residue side chain by increasing to the maximum the observation sensitivity to an NMR signal of the same amino acid residue side chain, and allowing NOE (nuclear Overhauser effect) between protons in the amino acid residue to be observed. The stable isotope-labeled aliphatic amino acid is for constituting a protein and satisfies all of the following conditions (1) to (3): (1) two or more carbon atoms are labeled with 13 C; (2) of two or more carbon atoms labeled with 13 C, a carbon atom other than a carbon atom of a methyl group, which is capable of bonding to a hydrogen atom, has one 1 H directly bonded thereto, while the carbon atom of the methyl group has at least one 1 H directly bonded thereto; and (3) other carbon atoms adjacent to all the 13 C are all 12 C.

First claim

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What is claimed is: 1. A stable isotope-labeled amino acid for constituting a protein, satisfying all of the following conditions (1) to (3): (1) two or more carbon atoms are labeled with 13 C; (2) the two or more carbon atoms labeled with 13 C include a carbon atom of a methyl group, a carbon atom other than the carbon atom of the methyl group, or a combination thereof, wherein the carbon atom other than the carbon atom of the methyl group has one 1 H directly bonded thereto…

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What does patent US9739732B2 cover?
Provided is a stable isotope-labeled aliphatic amino acid enabling the assignment of the signal of an amino acid residue side chain by increasing to the maximum the observation sensitivity to an NMR signal of the same amino acid residue side chain, and allowing NOE (nuclear Overhauser effect) between protons in the amino acid residue to be observed. The stable isotope-labeled aliphatic amino ac…
Who is the assignee on this patent?
Taiyo Nippon Sanso Corp
What technology area does this patent fall under?
Primary CPC classification G01N24/087. Mapped technology areas include Physics.
When was this patent published?
Publication date Tue Aug 22 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).