Compounds and methods for analysis and synthesis of saccharide compounds, and method for quantitating saccharide
US-2017299530-A1 · Oct 19, 2017 · US
US9739732B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9739732-B2 |
| Application number | US-201314421814-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 18, 2013 |
| Priority date | Aug 16, 2012 |
| Publication date | Aug 22, 2017 |
| Grant date | Aug 22, 2017 |
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Provided is a stable isotope-labeled aliphatic amino acid enabling the assignment of the signal of an amino acid residue side chain by increasing to the maximum the observation sensitivity to an NMR signal of the same amino acid residue side chain, and allowing NOE (nuclear Overhauser effect) between protons in the amino acid residue to be observed. The stable isotope-labeled aliphatic amino acid is for constituting a protein and satisfies all of the following conditions (1) to (3): (1) two or more carbon atoms are labeled with 13 C; (2) of two or more carbon atoms labeled with 13 C, a carbon atom other than a carbon atom of a methyl group, which is capable of bonding to a hydrogen atom, has one 1 H directly bonded thereto, while the carbon atom of the methyl group has at least one 1 H directly bonded thereto; and (3) other carbon atoms adjacent to all the 13 C are all 12 C.
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What is claimed is: 1. A stable isotope-labeled amino acid for constituting a protein, satisfying all of the following conditions (1) to (3): (1) two or more carbon atoms are labeled with 13 C; (2) the two or more carbon atoms labeled with 13 C include a carbon atom of a methyl group, a carbon atom other than the carbon atom of the methyl group, or a combination thereof, wherein the carbon atom other than the carbon atom of the methyl group has one 1 H directly bonded thereto…
Chemistry & Metallurgy · mapped topic
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